A9NCS0 (BIOB_COXBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	COXBURSA331_A0933

Organism

Coxiella burnetii (strain RSA 331 / Henzerling II) [Complete proteome] [HAMAP]

Taxonomic identifier

360115 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381334
Sites
	Metal binding	52	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	56	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	96	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	127	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	187	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	259	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9NCS0 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: FDC79146CEC713D8	FASTA	321	36,252
	10 20 30 40 50 60 MKGRNWNQAS VAKLFELPFF ELLYKAYETH RSHFDVRDME LCTLSSIKTG TCPEDCAYCP 70 80 90 100 110 120 QSGHYKTDVE REKLINLEAV LEQAKVAKEN GARRFCMGAA WRSPPKRELP KVLEMIKSVK 130 140 150 160 170 180 ALGLETCVTL GMLDQEQALQ LKEAGLDFYN HNLDTSPEFY KKIITTRTYQ DRMETLKNVR 190 200 210 220 230 240 NAGINVCCGG ILGMGESRAD RIQLLLELYQ LPEPPTSIPI NQLIPIKGTP LENTKAIDPF 250 260 270 280 290 300 EFIKTIAITR LLFPTSVIRL SAGREAMSDE LQAWCFMAGA NSIFYGDKLL TAKNPGQNRD 310 320 VNLLKKLGLK VPVLTEEYAC Y

References

[1]	"Genome sequencing of phylogenetically and phenotypically diverse Coxiella burnetii isolates." Seshadri R., Samuel J.E. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 331 / Henzerling II.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000890 Genomic DNA. Translation: ABX77241.1.
RefSeq	YP_001596710.1. NC_010117.1.
3D structure databases
ProteinModelPortal	A9NCS0.
SMR	A9NCS0. Positions 4-310.
ModBase	Search...
Protein-protein interaction databases
STRING	360115.COXBURSA331_A0933.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX77241; ABX77241; COXBURSA331_A0933.
GeneID	5792907.
KEGG	cbs:COXBURSA331_A0933.
PATRIC	17926121. VBICoxBur33747_0939.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239957.
KO	K01012.
OMA	RIMMPAS.
ProtClustDB	CLSK2390984.
Enzyme and pathway databases
BioCyc	CBUR360115:GI0X-957-MONOMER.
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_COXBR

Accession

Primary (citable) accession number: A9NCS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program