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A9NCS0

- BIOB_COXBR

UniProt

A9NCS0 - BIOB_COXBR

Protein

Biotin synthase

Gene

bioB

Organism
Coxiella burnetii (strain RSA 331 / Henzerling II)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi52 – 521Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi96 – 961Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi127 – 1271Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi187 – 1871Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi259 – 2591Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCBUR360115:GI0X-940-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:COXBURSA331_A0933
    OrganismiCoxiella burnetii (strain RSA 331 / Henzerling II)
    Taxonomic identifieri360115 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
    ProteomesiUP000008557: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 321321Biotin synthasePRO_0000381334Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi360115.COXBURSA331_A0933.

    Structurei

    3D structure databases

    ProteinModelPortaliA9NCS0.
    SMRiA9NCS0. Positions 4-310.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A9NCS0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKGRNWNQAS VAKLFELPFF ELLYKAYETH RSHFDVRDME LCTLSSIKTG    50
    TCPEDCAYCP QSGHYKTDVE REKLINLEAV LEQAKVAKEN GARRFCMGAA 100
    WRSPPKRELP KVLEMIKSVK ALGLETCVTL GMLDQEQALQ LKEAGLDFYN 150
    HNLDTSPEFY KKIITTRTYQ DRMETLKNVR NAGINVCCGG ILGMGESRAD 200
    RIQLLLELYQ LPEPPTSIPI NQLIPIKGTP LENTKAIDPF EFIKTIAITR 250
    LLFPTSVIRL SAGREAMSDE LQAWCFMAGA NSIFYGDKLL TAKNPGQNRD 300
    VNLLKKLGLK VPVLTEEYAC Y 321
    Length:321
    Mass (Da):36,252
    Last modified:February 5, 2008 - v1
    Checksum:iFDC79146CEC713D8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000890 Genomic DNA. Translation: ABX77241.1.
    RefSeqiYP_001596710.1. NC_010117.1.

    Genome annotation databases

    EnsemblBacteriaiABX77241; ABX77241; COXBURSA331_A0933.
    GeneIDi5792907.
    KEGGicbs:COXBURSA331_A0933.
    PATRICi17926121. VBICoxBur33747_0939.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000890 Genomic DNA. Translation: ABX77241.1 .
    RefSeqi YP_001596710.1. NC_010117.1.

    3D structure databases

    ProteinModelPortali A9NCS0.
    SMRi A9NCS0. Positions 4-310.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 360115.COXBURSA331_A0933.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABX77241 ; ABX77241 ; COXBURSA331_A0933 .
    GeneIDi 5792907.
    KEGGi cbs:COXBURSA331_A0933.
    PATRICi 17926121. VBICoxBur33747_0939.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci CBUR360115:GI0X-940-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequencing of phylogenetically and phenotypically diverse Coxiella burnetii isolates."
      Seshadri R., Samuel J.E.
      Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RSA 331 / Henzerling II.

    Entry informationi

    Entry nameiBIOB_COXBR
    AccessioniPrimary (citable) accession number: A9NCS0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3