A9NBY3 (FABH_COXBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.180

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	COXBURSA331_A0602

Organism

Coxiella burnetii (strain RSA 331 / Henzerling II) [Complete proteome] [HAMAP]

Taxonomic identifier

360115 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm Probable HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000088309
Regions
	Region	247 – 251	5	ACP-binding By similarity
Sites
	Active site	115	1	By similarity
	Active site	246	1	By similarity
	Active site	276	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9NBY3 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: FF85294CDBC55793	FASTA	319	34,687
	10 20 30 40 50 60 MTYARIQGVG SYIPQQILSN ADLEKMVNTT DEWIMQRVGV RERHVIANSP DNTTTMAVDA 70 80 90 100 110 120 AKRAIEMAGI DPAVIDMIIV GTATAEYYFP STACLVQKHL NLREDIPAFD INAACAGFVY 130 140 150 160 170 180 ALSIADQYIR NEGAKHILVI GVDSLTKVVD WKDRSTCILF GDGAGAVILQ AHKEPGILNT 190 200 210 220 230 240 ILHANGDYSD LITAKSGVWE RESVPHLHMY GKEVFKLAVT KLGEIVDEII EKSGLKQSGI 250 260 270 280 290 300 DWLIPHQANL RIIEATAKRL GLPRERVILT IEQHGNTSAA SIPLALDAAV RAGKIKRGDT 310 LLLEAFGAGL AWGAALLKL

References

[1]	"Genome sequencing of phylogenetically and phenotypically diverse Coxiella burnetii isolates." Seshadri R., Samuel J.E. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 331 / Henzerling II.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000890 Genomic DNA. Translation: ABX78580.1.
RefSeq	YP_001596423.1. NC_010117.1.
3D structure databases
ProteinModelPortal	A9NBY3.
SMR	A9NBY3. Positions 3-319.
ModBase	Search...
Protein-protein interaction databases
STRING	A9NBY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5793574.
GenomeReviews	Gene locus fabH in contig CP000890_GR.
KEGG	cbs:COXBURSA331_A0602.
PATRIC	17925459. VBICoxBur33747_0621.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649927.
OMA	DVNTACS.
ProtClustDB	PRK09352.
Enzyme and pathway databases
BioCyc	CBUR360115:COXBURSA331_A0602-MONOMER.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_COXBR

Accession

Primary (citable) accession number: A9NBY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program