A9NB28 (DUT_COXBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	COXBURSA331_A0398

Organism

Coxiella burnetii (strain RSA 331 / Henzerling II) [Complete proteome] [HAMAP]

Taxonomic identifier

360115 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116		PRO_1000076057
Regions
	Region	71 – 73	3	Substrate binding By similarity
	Region	88 – 90	3	Substrate binding By similarity
Sites
	Binding site	84	1	Substrate By similarity
	Binding site	98	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9NB28 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 00041727C1882C57	FASTA	152	16,214
	10 20 30 40 50 60 MTHSVQLKIL DKRLGSEFPL PAYATTGSAG LDLRACLDEP LKIEPDETCL ISTGLAIYLG 70 80 90 100 110 120 HSNVAATILP RSGLGHKHGI VLGNLVGLID SDYQGPLMVS CWNRGKEPYT INPGDRIAQL 130 140 150 VVLPILKAQF AVVEEFELTE RGAGGFGSSG QN

References

[1]	"Genome sequencing of phylogenetically and phenotypically diverse Coxiella burnetii isolates." Seshadri R., Samuel J.E. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 331 / Henzerling II.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000890 Genomic DNA. Translation: ABX79021.1.
RefSeq	YP_001596243.1. NC_010117.1.
3D structure databases
ProteinModelPortal	A9NB28.
SMR	A9NB28. Positions 1-139.
ModBase	Search...
Protein-protein interaction databases
STRING	360115.COXBURSA331_A0398.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX79021; ABX79021; COXBURSA331_A0398.
GeneID	5793843.
KEGG	cbs:COXBURSA331_A0398.
PATRIC	17925056. VBICoxBur33747_0422.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HOG000028968.
KO	K01520.
OMA	ILPRSGM.
ProtClustDB	PRK00601.
Enzyme and pathway databases
BioCyc	CBUR360115:GI0X-403-MONOMER.
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact.
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_COXBR

Accession

Primary (citable) accession number: A9NB28

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	May 29, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program