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Protein

N-acetylneuraminate epimerase

Gene

nanM

Organism
Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers. Probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in the beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses.UniRule annotation

Catalytic activityi

N-acetyl-alpha-neuraminate = N-acetyl-beta-neuraminate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei241Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciSENT28901:GH9O-2405-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate epimeraseUniRule annotation (EC:5.1.3.24UniRule annotation)
Alternative name(s):
N-acetylneuraminate mutarotaseUniRule annotation
Short name:
Neu5Ac mutarotaseUniRule annotation
Sialic acid epimeraseUniRule annotation
Gene namesi
Name:nanMUniRule annotation
Ordered Locus Names:SPAB_02408
OrganismiSalmonella paratyphi B (strain ATCC BAA-1250 / SPB7)
Taxonomic identifieri1016998 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29UniRule annotationAdd BLAST29
ChainiPRO_000033306630 – 386N-acetylneuraminate epimeraseAdd BLAST357

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati51 – 95Kelch 1Add BLAST45
Repeati97 – 149Kelch 2Add BLAST53
Repeati151 – 186Kelch 3Add BLAST36
Repeati187 – 232Kelch 4Add BLAST46
Repeati235 – 284Kelch 5Add BLAST50
Repeati306 – 355Kelch 6Add BLAST50
Repeati357 – 386Kelch 7Add BLAST30

Sequence similaritiesi

Belongs to the NanM family.UniRule annotation
Contains 7 Kelch repeats.UniRule annotation

Keywords - Domaini

Kelch repeat, Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000218207.
KOiK17948.

Family and domain databases

Gene3Di2.120.10.80. 2 hits.
HAMAPiMF_01195. NanM. 1 hit.
InterProiIPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR019936. Mutatrotase_YjhT-like.
[Graphical view]
PfamiPF01344. Kelch_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03547. muta_rot_YjhT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A9N6P2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMQMKNFKK MMTLMALCLS VAITTSGYAT TLPDIPEPLK NGTGAIDNNG
60 70 80 90 100
VIYVGLGTAG TSWYKIDLKK QHKDWERIKS FPGGAREQSV SVFLNDELYV
110 120 130 140 150
FGGVGKKNSE SPLQVYSDVY KYSPVKNTWQ KVDTISPVGL TGHTGVKLNE
160 170 180 190 200
TMVLITGGVN EHIFDKYFID IAAAAADESE KNKVIYNYFN KPAKDYFFNK
210 220 230 240 250
IVFIYNAKEN TWKNAGELPD AGTAGSSSVM ENNFLMLING ELKPGLRTDV
260 270 280 290 300
IYRAMWDNDK LTWLKNSQLP PSPGEQQQEG LAGAFSGYSH GVLLVGGGAN
310 320 330 340 350
FPGAKQNYTN GKFYSHEGIN KKWRDEVYGL VNGHWQYMGK MKQPLGYGVS
360 370 380
VSYGDEVFLI GGENAKGKPV SSVTSFTMRD GNLLIK
Length:386
Mass (Da):42,573
Last modified:May 20, 2008 - v2
Checksum:iBFD37D669F11944F
GO

Sequence cautioni

The sequence ABX67789 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000886 Genomic DNA. Translation: ABX67789.1. Different initiation.
RefSeqiWP_000525756.1. NC_010102.1.

Genome annotation databases

EnsemblBacteriaiABX67789; ABX67789; SPAB_02408.
KEGGispq:SPAB_02408.
PATRICi18532166. VBISalEnt120821_1953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000886 Genomic DNA. Translation: ABX67789.1. Different initiation.
RefSeqiWP_000525756.1. NC_010102.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX67789; ABX67789; SPAB_02408.
KEGGispq:SPAB_02408.
PATRICi18532166. VBISalEnt120821_1953.

Phylogenomic databases

HOGENOMiHOG000218207.
KOiK17948.

Enzyme and pathway databases

BioCyciSENT28901:GH9O-2405-MONOMER.

Family and domain databases

Gene3Di2.120.10.80. 2 hits.
HAMAPiMF_01195. NanM. 1 hit.
InterProiIPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR019936. Mutatrotase_YjhT-like.
[Graphical view]
PfamiPF01344. Kelch_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03547. muta_rot_YjhT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNANM_SALPB
AccessioniPrimary (citable) accession number: A9N6P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: November 2, 2016
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.