Reviewed, UniProtKB/Swiss-Prot A9N601 (RLMG_SALPB)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	SPAB_04019

Organism

Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) [Complete proteome] [HAMAP]

Taxonomic identifier

272994 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella enterica subsp. enterica serovar Paratyphi B

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366499

Sequences

	Sequence		Length	Mass (Da)
	A9N601-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 56B702A6AEB4A894	FASTA	378	42,300
	10 20 30 40 50 60 MSHVDDGFRS LTLKRFPQTD DVNPLLAWEA ADEYLLQQLD ETEIRGPVLI LNDTFGALSC 70 80 90 100 110 120 ALAEHSPYSI GDSYLSELGT RENLRHNGIA ESSVTFLDST ADYPQAPGVV LIKVPKTLAL 130 140 150 160 170 180 LEQQLRALRK VVTVQTRIIA GAKARDIHTS TLELFEKVLG PTTTTLAWKK ARLINCTFSH 190 200 210 220 230 240 PQLADAPQTL SWKLEDTGWT IHNHANVFSR TGLDIGARFF MQHLPENLDG EIVDLGCGNG 250 260 270 280 290 300 VIGLSLLAKN PQANVVFVDE SPMAVDSSRL NVETNLPEAF ERCEFMINNA LSGVEPFRFN 310 320 330 340 350 360 AVFCNPPFHQ KHALTDNIAW EMFHHARRCL KINGELYIVA NRHLDYFHKL KKIFGNCATI 370 ATNNKFVILK AVKQGRRR

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton R., Cordes M., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000886 Genomic DNA. Translation: ABX69347.1.
RefSeq	YP_001590180.1.
3D structure databases
SMR	A9N601. Positions 27-372.
ModBase	Search...
Genome annotation databases
GeneID	5778467.
GenomeReviews	Gene locus SPAB_04019 in contig CP000886_GR.
KEGG	spq:SPAB_04019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG321931.
OMA	CGNGVLG.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SALPB

Accession

Primary (citable) accession number: A9N601

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 5, 2008
Last modified:	January 19, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)