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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei118Important for catalytic activityUniRule annotation1
Sitei140Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:SPAB_00579
OrganismiSalmonella paratyphi B (strain ATCC BAA-1250 / SPB7)
Taxonomic identifieri1016998 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000880621 – 715Fatty acid oxidation complex subunit alphaAdd BLAST715

Proteomic databases

PRIDEiA9N453

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA9N453
SMRiA9N453
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190Enoyl-CoA hydrataseUniRule annotationAdd BLAST190
Regioni306 – 7153-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST410

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000261346
KOiK01782
OMAiPFRYMDT

Family and domain databases

HAMAPiMF_01617 FadJ, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012802 FadJ
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 1 hit
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02440 FadJ, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit

Sequencei

Sequence statusi: Complete.

A9N453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTSAFMLN VRLDNVAVVA IDVPGEKVNT LKAEFAAQVR AILKQIRENK
60 70 80 90 100
ALQGVVFISA KADNFIAGAD INMIGHCQNA QEAETLARQG QQLMAEIQAL
110 120 130 140 150
PVPVIAAIHG ACLGGGLEMA LACHRRICTD DVKTVLGLPE VQLGLLPGSG
160 170 180 190 200
GTQRLPRLVG VSTALDMILT GKQLRARQAL KAGLVDDVVP QTILLEAAVE
210 220 230 240 250
LAKKERLAQR TLPVRERILA GPLGRALLFR LVRKKTAQKT QGNYPATERI
260 270 280 290 300
IDVIETGLAQ GSSSGYDAEA RAFGELAMTP QSQALRAIFF ASTEVKKDPG
310 320 330 340 350
SDAPPGPLNS VGILGGGLMG GGIAWVTACK GGLPVRIKDI NTQGINHALK
360 370 380 390 400
YSWDLLETKV RRRHIKASER DKQLALISGS TDYRGFSHRD LVIEAVFEDL
410 420 430 440 450
PLKQQMVAEV EQNCAAHTIF ASNTSSLPIG DIAANAARPE QVIGLHFFSP
460 470 480 490 500
VEKMPLVEVI PHASTSAQTI ATTVKLAKKQ GKTPIVVSDK AGFYVNRILA
510 520 530 540 550
PYINEAIRML TEGERVEHID AALVKFGFPV GPIQLLDEVG IDTGTKIIPV
560 570 580 590 600
LEAAYGERFS APANVVASIL NDDRKGRKNG RGFYLYGEKG RKSKKQVDPA
610 620 630 640 650
IYKLIGVQGQ SRLSAQQVAE RCVMLMLNEA ARCFDEKVIR SARDGDIGAV
660 670 680 690 700
FGIGFPPFLG GPFRYMDALG PGEMVATLQR LAALYGPRYA PCEQLVRMAE
710
RREHFWTNGE TDQGN
Length:715
Mass (Da):77,225
Last modified:February 5, 2008 - v1
Checksum:iA17B4AAA84BBE4FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000886 Genomic DNA Translation: ABX66005.1
RefSeqiWP_000214135.1, NC_010102.1

Genome annotation databases

EnsemblBacteriaiABX66005; ABX66005; SPAB_00579
KEGGispq:SPAB_00579
PATRICifig|1016998.12.peg.543

Similar proteinsi

Entry informationi

Entry nameiFADJ_SALPB
AccessioniPrimary (citable) accession number: A9N453
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: February 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program