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Protein

Endonuclease V

Gene

nfi

Organism
Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi35 – 351MagnesiumUniRule annotation
Metal bindingi103 – 1031MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSENT28901:GH9O-5147-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease VUniRule annotation (EC:3.1.21.7UniRule annotation)
Alternative name(s):
Deoxyinosine 3'endonucleaseUniRule annotation
Deoxyribonuclease VUniRule annotation
Short name:
DNase VUniRule annotation
Gene namesi
Name:nfiUniRule annotation
Ordered Locus Names:SPAB_05158
OrganismiSalmonella paratyphi B (strain ATCC BAA-1250 / SPB7)
Taxonomic identifieri1016998 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Endonuclease VPRO_1000083699Add
BLAST

Interactioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei73 – 731Interaction with target DNAUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA9N0L0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the endonuclease V family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229135.
KOiK05982.
OMAiREYPALM.
OrthoDBiEOG6C0163.

Family and domain databases

HAMAPiMF_00801. Endonuclease_5.
InterProiIPR007581. Endonuclease-V.
[Graphical view]
PfamiPF04493. Endonuclease_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9N0L0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLASLRAQQ IELASSVCRE DRLDKDPPAF IGGADVGFEQ GGEVTRAAMV
60 70 80 90 100
LLKYPSLELV EYKVARIATT MPYIPGFLSF REYPALLAAW EQLSQKPDLL
110 120 130 140 150
FVDGHGISHP RRLGVASHFG LLVDVPTIGV AKKRLCGKFE PLSTEPGALS
160 170 180 190 200
PLMDKGEQLA WVWRSKARCN PLFIATGHRV STDSALAWVQ RCMKGYRLPE
210 220
PTRWADAVAS GRPAFVRWQE IQR
Length:223
Mass (Da):24,776
Last modified:February 5, 2008 - v1
Checksum:i1B33205154954E40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000886 Genomic DNA. Translation: ABX70439.1.
RefSeqiWP_000362363.1. NC_010102.1.

Genome annotation databases

EnsemblBacteriaiABX70439; ABX70439; SPAB_05158.
KEGGispq:SPAB_05158.
PATRICi18536735. VBISalEnt120821_4172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000886 Genomic DNA. Translation: ABX70439.1.
RefSeqiWP_000362363.1. NC_010102.1.

3D structure databases

ProteinModelPortaliA9N0L0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX70439; ABX70439; SPAB_05158.
KEGGispq:SPAB_05158.
PATRICi18536735. VBISalEnt120821_4172.

Phylogenomic databases

HOGENOMiHOG000229135.
KOiK05982.
OMAiREYPALM.
OrthoDBiEOG6C0163.

Enzyme and pathway databases

BioCyciSENT28901:GH9O-5147-MONOMER.

Family and domain databases

HAMAPiMF_00801. Endonuclease_5.
InterProiIPR007581. Endonuclease-V.
[Graphical view]
PfamiPF04493. Endonuclease_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1250 / SPB7.

Entry informationi

Entry nameiNFI_SALPB
AccessioniPrimary (citable) accession number: A9N0L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: November 11, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.