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A9MQ82 (BGAL_SALAR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:SARI_02832
OrganismSalmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) [Complete proteome] [HAMAP]
Taxonomic identifier41514 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length1025 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Sequence caution

The sequence ABX22680.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10251025Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000367007

Regions

Region538 – 5414Substrate binding By similarity

Sites

Active site4621Proton donor By similarity
Active site5381Nucleophile By similarity
Metal binding2021Sodium By similarity
Metal binding4171Magnesium 1 By similarity
Metal binding4191Magnesium 1 By similarity
Metal binding4621Magnesium 1 By similarity
Metal binding5981Magnesium 2 By similarity
Metal binding6021Sodium; via carbonyl oxygen By similarity
Metal binding6051Sodium By similarity
Binding site1031Substrate By similarity
Binding site2021Substrate By similarity
Binding site4621Substrate By similarity
Binding site6051Substrate By similarity
Binding site10031Substrate By similarity
Site3581Transition state stabilizer By similarity
Site3921Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A9MQ82 [UniParc].

Last modified March 24, 2009. Version 2.
Checksum: E76A52C51D8A95B7

FASTA1,025117,238
        10         20         30         40         50         60 
MTPERDSLAA VLARRDWENP AVTQFNRLTA HPPFCSWRKA DDAQRNQYAA QIRSLNGVWK 

        70         80         90        100        110        120 
FAWFSSPQAV PENWRLEDLT EAGTINVPSN WQMYGYDSPI YSNITYPFPV NPPCVPAENP 

       130        140        150        160        170        180 
TGCYSLTFCM DDDWLTEGQT RIIFDGVNSA FHLWCNGRWV GYGQDSRLPS EFDLSEYLQV 

       190        200        210        220        230        240 
GENRLAVLVL RWSDGSYLED QDMWRMSGIF RDVSLLHKPT TQITDFHLHT HLNDDFTQAV 

       250        260        270        280        290        300 
LEAEVRLAGG VNDDLQLVLH LWQGETLAGE ARATPGSEII DERGGYDDRA TLRLNINRPA 

       310        320        330        340        350        360 
LWSAETPNLY RAVIQLRTAD GELIEAEACD VGFRQVRIDK GLLLLNGKPL LIRGTNRHEH 

       370        380        390        400        410        420 
HPERGQVMDY DTMVQDILLM KQNNFNAVRC SHYPNHPQWY ALCDRYGLYV VDEANIETHG 

       430        440        450        460        470        480 
MTPMNRLSDD PDWLPAMSQR VTRMVQRDRN HPSIIIWSLG NESGHGANHD ALYRWLKAED 

       490        500        510        520        530        540 
PSRPVQYEGG GADTAATDII CPMYARVDQD QPFPAVPKWS IKKWLSLPGE QRPLILCEYA 

       550        560        570        580        590        600 
HAMGNSFGGF AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD ADGKPWSAYG GDFGDTPNDR 

       610        620        630        640        650        660 
QFCMNGLVFA DRTPHPALYE AKHVQQFFQF RLLPGEERRI EVQSEYLFRH SDNEILRWML 

       670        680        690        700        710        720 
AQEGNQLASG EVVLDIAPQG RQIILLPAFP QPETAGQLWL TVRVEQPLAT SWSEAGHISA 

       730        740        750        760        770        780 
WQQWPLEEKL CVSKPTRASV APVLTMRDGE FCVTQGNLRW QFCRQQGWLT QFWRDDEAQL 

       790        800        810        820        830        840 
LTPLIDQFTR APLDNDIGVS EATRIDPNAW VERWKAAGHY CAEPALLLCD ADELADAVLI 

       850        860        870        880        890        900 
TTAHAWQYQG ATLFISRKTY RIDDHGEMQI DIGVEVASGM PYPARIGLSC QLAQVNERVE 

       910        920        930        940        950        960 
WLGLGPHENY PDRLSSACFD RWNLPLDAMY TPYVFPTENG LRCGTRQLRY GAHQWSGDFQ 

       970        980        990       1000       1010       1020 
FNISRYSQRQ LMETSHRHLL QAEAGVWLNI DGYHMGVGGD DSWSPSVSPE FQLSARHYHY 


QIAWK 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000880 Genomic DNA. Translation: ABX22680.1. Different initiation.

3D structure databases

ProteinModelPortalA9MQ82.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING41514.SARI_02832.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX22680; ABX22680; SARI_02832.
PATRIC18474706. VBISalEnt13497_2701.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
OrthoDBEOG6XWV0T.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_SALAR
AccessionPrimary (citable) accession number: A9MQ82
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 42 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries