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A9MQ82

- BGAL_SALAR

UniProt

A9MQ82 - BGAL_SALAR

Protein

Beta-galactosidase

Gene

lacZ

Organism
Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 2 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei103 – 1031SubstrateUniRule annotation
    Metal bindingi202 – 2021SodiumUniRule annotation
    Binding sitei202 – 2021SubstrateUniRule annotation
    Sitei358 – 3581Transition state stabilizerUniRule annotation
    Sitei392 – 3921Transition state stabilizerUniRule annotation
    Metal bindingi417 – 4171Magnesium 1UniRule annotation
    Metal bindingi419 – 4191Magnesium 1UniRule annotation
    Active sitei462 – 4621Proton donorUniRule annotation
    Metal bindingi462 – 4621Magnesium 1UniRule annotation
    Binding sitei462 – 4621SubstrateUniRule annotation
    Active sitei538 – 5381NucleophileUniRule annotation
    Metal bindingi598 – 5981Magnesium 2UniRule annotation
    Metal bindingi602 – 6021Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi605 – 6051SodiumUniRule annotation
    Binding sitei605 – 6051SubstrateUniRule annotation
    Binding sitei1003 – 10031SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:SARI_02832
    OrganismiSalmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)
    Taxonomic identifieri41514 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000002084: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10251025Beta-galactosidasePRO_0000367007Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi41514.SARI_02832.

    Structurei

    3D structure databases

    ProteinModelPortaliA9MQ82.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni538 – 5414Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A9MQ82-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPERDSLAA VLARRDWENP AVTQFNRLTA HPPFCSWRKA DDAQRNQYAA     50
    QIRSLNGVWK FAWFSSPQAV PENWRLEDLT EAGTINVPSN WQMYGYDSPI 100
    YSNITYPFPV NPPCVPAENP TGCYSLTFCM DDDWLTEGQT RIIFDGVNSA 150
    FHLWCNGRWV GYGQDSRLPS EFDLSEYLQV GENRLAVLVL RWSDGSYLED 200
    QDMWRMSGIF RDVSLLHKPT TQITDFHLHT HLNDDFTQAV LEAEVRLAGG 250
    VNDDLQLVLH LWQGETLAGE ARATPGSEII DERGGYDDRA TLRLNINRPA 300
    LWSAETPNLY RAVIQLRTAD GELIEAEACD VGFRQVRIDK GLLLLNGKPL 350
    LIRGTNRHEH HPERGQVMDY DTMVQDILLM KQNNFNAVRC SHYPNHPQWY 400
    ALCDRYGLYV VDEANIETHG MTPMNRLSDD PDWLPAMSQR VTRMVQRDRN 450
    HPSIIIWSLG NESGHGANHD ALYRWLKAED PSRPVQYEGG GADTAATDII 500
    CPMYARVDQD QPFPAVPKWS IKKWLSLPGE QRPLILCEYA HAMGNSFGGF 550
    AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD ADGKPWSAYG GDFGDTPNDR 600
    QFCMNGLVFA DRTPHPALYE AKHVQQFFQF RLLPGEERRI EVQSEYLFRH 650
    SDNEILRWML AQEGNQLASG EVVLDIAPQG RQIILLPAFP QPETAGQLWL 700
    TVRVEQPLAT SWSEAGHISA WQQWPLEEKL CVSKPTRASV APVLTMRDGE 750
    FCVTQGNLRW QFCRQQGWLT QFWRDDEAQL LTPLIDQFTR APLDNDIGVS 800
    EATRIDPNAW VERWKAAGHY CAEPALLLCD ADELADAVLI TTAHAWQYQG 850
    ATLFISRKTY RIDDHGEMQI DIGVEVASGM PYPARIGLSC QLAQVNERVE 900
    WLGLGPHENY PDRLSSACFD RWNLPLDAMY TPYVFPTENG LRCGTRQLRY 950
    GAHQWSGDFQ FNISRYSQRQ LMETSHRHLL QAEAGVWLNI DGYHMGVGGD 1000
    DSWSPSVSPE FQLSARHYHY QIAWK 1025
    Length:1,025
    Mass (Da):117,238
    Last modified:March 24, 2009 - v2
    Checksum:iE76A52C51D8A95B7
    GO

    Sequence cautioni

    The sequence ABX22680.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000880 Genomic DNA. Translation: ABX22680.1. Different initiation.

    Genome annotation databases

    EnsemblBacteriaiABX22680; ABX22680; SARI_02832.
    PATRICi18474706. VBISalEnt13497_2701.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000880 Genomic DNA. Translation: ABX22680.1 . Different initiation.

    3D structure databases

    ProteinModelPortali A9MQ82.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 41514.SARI_02832.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABX22680 ; ABX22680 ; SARI_02832 .
    PATRICi 18474706. VBISalEnt13497_2701.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    OrthoDBi EOG6XWV0T.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-731 / CDC346-86 / RSK2980.

    Entry informationi

    Entry nameiBGAL_SALAR
    AccessioniPrimary (citable) accession number: A9MQ82
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 43 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3