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A9MQ82

- BGAL_SALAR

UniProt

A9MQ82 - BGAL_SALAR

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031SubstrateUniRule annotation
Metal bindingi202 – 2021SodiumUniRule annotation
Binding sitei202 – 2021SubstrateUniRule annotation
Sitei358 – 3581Transition state stabilizerUniRule annotation
Sitei392 – 3921Transition state stabilizerUniRule annotation
Metal bindingi417 – 4171Magnesium 1UniRule annotation
Metal bindingi419 – 4191Magnesium 1UniRule annotation
Active sitei462 – 4621Proton donorUniRule annotation
Metal bindingi462 – 4621Magnesium 1UniRule annotation
Binding sitei462 – 4621SubstrateUniRule annotation
Active sitei538 – 5381NucleophileUniRule annotation
Metal bindingi598 – 5981Magnesium 2UniRule annotation
Metal bindingi602 – 6021Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi605 – 6051SodiumUniRule annotation
Binding sitei605 – 6051SubstrateUniRule annotation
Binding sitei1003 – 10031SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:SARI_02832
OrganismiSalmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)
Taxonomic identifieri41514 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000002084: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10251025Beta-galactosidasePRO_0000367007Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi41514.SARI_02832.

Structurei

3D structure databases

ProteinModelPortaliA9MQ82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni538 – 5414Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9MQ82-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTPERDSLAA VLARRDWENP AVTQFNRLTA HPPFCSWRKA DDAQRNQYAA
60 70 80 90 100
QIRSLNGVWK FAWFSSPQAV PENWRLEDLT EAGTINVPSN WQMYGYDSPI
110 120 130 140 150
YSNITYPFPV NPPCVPAENP TGCYSLTFCM DDDWLTEGQT RIIFDGVNSA
160 170 180 190 200
FHLWCNGRWV GYGQDSRLPS EFDLSEYLQV GENRLAVLVL RWSDGSYLED
210 220 230 240 250
QDMWRMSGIF RDVSLLHKPT TQITDFHLHT HLNDDFTQAV LEAEVRLAGG
260 270 280 290 300
VNDDLQLVLH LWQGETLAGE ARATPGSEII DERGGYDDRA TLRLNINRPA
310 320 330 340 350
LWSAETPNLY RAVIQLRTAD GELIEAEACD VGFRQVRIDK GLLLLNGKPL
360 370 380 390 400
LIRGTNRHEH HPERGQVMDY DTMVQDILLM KQNNFNAVRC SHYPNHPQWY
410 420 430 440 450
ALCDRYGLYV VDEANIETHG MTPMNRLSDD PDWLPAMSQR VTRMVQRDRN
460 470 480 490 500
HPSIIIWSLG NESGHGANHD ALYRWLKAED PSRPVQYEGG GADTAATDII
510 520 530 540 550
CPMYARVDQD QPFPAVPKWS IKKWLSLPGE QRPLILCEYA HAMGNSFGGF
560 570 580 590 600
AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD ADGKPWSAYG GDFGDTPNDR
610 620 630 640 650
QFCMNGLVFA DRTPHPALYE AKHVQQFFQF RLLPGEERRI EVQSEYLFRH
660 670 680 690 700
SDNEILRWML AQEGNQLASG EVVLDIAPQG RQIILLPAFP QPETAGQLWL
710 720 730 740 750
TVRVEQPLAT SWSEAGHISA WQQWPLEEKL CVSKPTRASV APVLTMRDGE
760 770 780 790 800
FCVTQGNLRW QFCRQQGWLT QFWRDDEAQL LTPLIDQFTR APLDNDIGVS
810 820 830 840 850
EATRIDPNAW VERWKAAGHY CAEPALLLCD ADELADAVLI TTAHAWQYQG
860 870 880 890 900
ATLFISRKTY RIDDHGEMQI DIGVEVASGM PYPARIGLSC QLAQVNERVE
910 920 930 940 950
WLGLGPHENY PDRLSSACFD RWNLPLDAMY TPYVFPTENG LRCGTRQLRY
960 970 980 990 1000
GAHQWSGDFQ FNISRYSQRQ LMETSHRHLL QAEAGVWLNI DGYHMGVGGD
1010 1020
DSWSPSVSPE FQLSARHYHY QIAWK
Length:1,025
Mass (Da):117,238
Last modified:March 24, 2009 - v2
Checksum:iE76A52C51D8A95B7
GO

Sequence cautioni

The sequence ABX22680.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000880 Genomic DNA. Translation: ABX22680.1. Different initiation.

Genome annotation databases

EnsemblBacteriaiABX22680; ABX22680; SARI_02832.
PATRICi18474706. VBISalEnt13497_2701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000880 Genomic DNA. Translation: ABX22680.1 . Different initiation.

3D structure databases

ProteinModelPortali A9MQ82.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 41514.SARI_02832.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX22680 ; ABX22680 ; SARI_02832 .
PATRICi 18474706. VBISalEnt13497_2701.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
OrthoDBi EOG6XWV0T.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-731 / CDC346-86 / RSK2980.

Entry informationi

Entry nameiBGAL_SALAR
AccessioniPrimary (citable) accession number: A9MQ82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: November 26, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3