Reviewed, UniProtKB/Swiss-Prot A9MH27 (GHRA_SALAR)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase A
    EC=1.1.1.79
    EC=1.1.1.81
Alternative name(s):
    2-ketoacid reductase

Gene names

Name:	ghrA
Ordered Locus Names:	SARI_01863

Organism

Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) [Complete proteome] [HAMAP]

Taxonomic identifier

41514 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01666
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01666 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01666
Subcellular location	Cytoplasm Probable HAMAP MF_01666.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Glyoxylate/hydroxypyruvate reductase A HAMAP MF_01666		PRO_0000348367
Sites
	Active site	227	1	By similarity
	Active site	275	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9MH27-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: D960AB6E2EBAF63C	FASTA	312	35,130
	10 20 30 40 50 60 MEIIFYHPTF NAAWWVNALE KALPHVRVRE WKAGDNSPAD YALVWQPPVE MLAGRRLKAV 70 80 90 100 110 120 FALGAGVDSI LSELNTHPEM LDASIPLFRL EDTGMGLQMQ EYAVSQVLHW FRRFDDYQAL 130 140 150 160 170 180 KNQAIWKPLP EYTREEFSVG IMGAGILGSK VAESLQAWGF PLRCWSRSRK SWPGVESYAG 190 200 210 220 230 240 REELDAFLNQ TRVLINLLPN TAQTVGIINR ELLNKLPDSA YVLNLARGVH VNEADLLAAL 250 260 270 280 290 300 ESGKLKGAML DVFSQEPLPQ ESPLWRHSRV AMTPHIAAVT RPAEAIEYIS RTINQLERGE 310 PVTGQVDRAR GY

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton R., Chunyan W., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000880 Genomic DNA. Translation: ABX21747.1.
RefSeq	YP_001570888.1.
3D structure databases
SMR	A9MH27. Positions 1-283.
ModBase	Search...
Genome annotation databases
GeneID	5761154.
GenomeReviews	Gene locus SARI_01863 in contig CP000880_GR.
KEGG	ses:SARI_01863.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG731446.
OMA	YALVWRA.
Family and domain databases
HAMAP	MF_01666. 2-Hacid_dh_C_GhrA. [Tree]
InterPro	IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRA_SALAR

Accession

Primary (citable) accession number: A9MH27

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 5, 2008
Last modified:	January 19, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)