Reviewed, UniProtKB/Swiss-Prot A9MGK5 (NRFA_SALAR)

Names and origin

Protein names

Recommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase

Gene names

Name:	nrfA
Ordered Locus Names:	SARI_03399

Organism

Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) [Complete proteome] [HAMAP]

Taxonomic identifier

41514 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a role in nitrite reduction By similarity.
Catalytic activity	NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+).
Cofactor	Binds 1 calcium ion per monomer By similarity. Binds 5 heme groups covalently per monomer By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subcellular location	PeriplasmBy similarity.
Sequence similarities	Belongs to the cytochrome c-552 family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrogen compound metabolic process Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: HAMAP
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrite reductase (cytochrome, ammonia-forming) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 478	452	Cytochrome c-552		PRO_1000085448
Sites
	Metal binding	94	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	126	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	164	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	213	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	215	1	Calcium By similarity
	Metal binding	216	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	261	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	263	1	Calcium By similarity
	Metal binding	275	1	Iron (heme 5 axial ligand) By similarity
	Metal binding	286	1	Iron (heme 4 axial ligand) By similarity
	Metal binding	301	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	318	1	Iron (heme 5 axial ligand) By similarity
	Metal binding	393	1	Iron (heme 4 axial ligand) By similarity
	Binding site	122	1	Heme 1 (covalent) By similarity
	Binding site	125	1	Heme 1 (covalent) By similarity
	Binding site	160	1	Heme 2 (covalent) By similarity
	Binding site	163	1	Heme 2 (covalent) By similarity
	Binding site	209	1	Heme 3 (covalent) By similarity
	Binding site	212	1	Heme 3 (covalent) By similarity
	Binding site	216	1	Substrate By similarity
	Binding site	264	1	Substrate By similarity
	Binding site	282	1	Heme 4 (covalent) By similarity
	Binding site	285	1	Heme 4 (covalent) By similarity
	Binding site	314	1	Heme 5 (covalent) By similarity
	Binding site	317	1	Heme 5 (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9MGK5-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: D36B87B3A4052BB3	FASTA	478	53,779
	10 20 30 40 50 60 MARKTLRARR FFSLIFPFFF MTSVYAEQTS VSAKTVTVEA KNETFSPQHP DQYQSWKATS 70 80 90 100 110 120 EQSAREDALA EDPRLVILWA GYPFSRDYNK PRGHAYAVTD VRETLRTGAP KTAEEGPLPM 130 140 150 160 170 180 ACWSCKSPDV ARLIQQEGED GYFHGKWARG GPEIVNDLGC ADCHNTASDD FAQGKPALTL 190 200 210 220 230 240 SRPYAERAME AIGKPFDKAG RFDQQSMVCG QCHVEYYFEG KNKAVKFPWD EGMKVENMEK 250 260 270 280 290 300 YYDAIAFSDW TNSLSKTPML KAQHPEYETW SAGIHGKNNV TCIDCHMPKV QNAEGKLYTD 310 320 330 340 350 360 HKIGNPFDNF AQTCANCHTQ DKASLQKVVA ERKQAIHDLK IKVEDQLVHA HFEAKAAWDA 370 380 390 400 410 420 GATDAEMKPI LNDIRHAQWR WDLAIASHGI HMHAPEEGLR MLGSAMDKAA DARTKLARLL 430 440 450 460 470 ATKGITHEIP LPDISTKEKA QKAIGLNMQQ INAEKQDFLK TVVPQWEDQA RKNGLLSQ

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton R., Chunyan W., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000880 Genomic DNA. Translation: ABX23229.1.
RefSeq	YP_001572371.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5761056.
GenomeReviews	Gene locus SARI_03399 in contig CP000880_GR.
KEGG	ses:SARI_03399.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01182. [Tree]
InterPro	IPR003321. Cyt_c552. IPR017570. Cytc_552_NO2Rdtase_formate-dep. IPR011031. Multihaem_cyt. [Graphical view]
Pfam	PF02335. Cytochrom_C552. 1 hit. [Graphical view]
PIRSF	PIRSF000243. Cyt_c552. 1 hit.
PROSITE	PS51008. MULTIHEME_CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NRFA_SALAR

Accession

Primary (citable) accession number: A9MGK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	November 4, 2008
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)