Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 53ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:SARI_01668
OrganismiSalmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)
Taxonomic identifieri41514 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002084 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000775931 – 275NH(3)-dependent NAD(+) synthetaseAdd BLAST275

Proteomic databases

PRIDEiA9MFF4.

Interactioni

Protein-protein interaction databases

STRINGi882884.SARI_01668.

Structurei

3D structure databases

ProteinModelPortaliA9MFF4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RA1. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
KOiK01916.
OMAiAKIIEGW.
OrthoDBiPOG091H02ZG.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

A9MFF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLQQEIIQA LGAKSYINPE EEIRRSVDFL KAYLKTYPFL KSLVLGISGG
60 70 80 90 100
QDSTLAGKLS QMAISELREE TDDDALQFIA VRLPYGVQAD EQDCQDAIAF
110 120 130 140 150
IKPDRVLTVN IKGAVLASEQ ALREAGIELS DFVRGNEKAR ERMKAQYSIA
160 170 180 190 200
GMTHGVVVGT DHAAEAITGF FTKYGDGGTD INPLHRLNKR QGKQLLAALG
210 220 230 240 250
CPEHLYKKVP TADLEDDRPS LPDEAALGVT YDNIDDYLEG KTLAPAIAKI
260 270
IEGWYVKTEH KRRLPITVFD DFWKK
Length:275
Mass (Da):30,515
Last modified:February 5, 2008 - v1
Checksum:iAAC76EA89F8D4983
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000880 Genomic DNA. Translation: ABX21559.1.
RefSeqiWP_000174993.1. NC_010067.1.

Genome annotation databases

EnsemblBacteriaiABX21559; ABX21559; SARI_01668.
KEGGises:SARI_01668.
PATRICi18472431. VBISalEnt13497_1591.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000880 Genomic DNA. Translation: ABX21559.1.
RefSeqiWP_000174993.1. NC_010067.1.

3D structure databases

ProteinModelPortaliA9MFF4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi882884.SARI_01668.

Proteomic databases

PRIDEiA9MFF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX21559; ABX21559; SARI_01668.
KEGGises:SARI_01668.
PATRICi18472431. VBISalEnt13497_1591.

Phylogenomic databases

eggNOGiENOG4107RA1. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
KOiK01916.
OMAiAKIIEGW.
OrthoDBiPOG091H02ZG.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADE_SALAR
AccessioniPrimary (citable) accession number: A9MFF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.