Reviewed, UniProtKB/Swiss-Prot A9MCV8 (TRUA_BRUC2)

Names and origin

Protein names

Recommended name:
    tRNA pseudouridine synthase A
    EC=5.4.99.12
Alternative name(s):
    tRNA-uridine isomerase I
    tRNA pseudouridylate synthase I

Gene names

Name:	truA
Ordered Locus Names:	BCAN_B1053

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity.
Catalytic activity	tRNA uridine = tRNA pseudouridine. HAMAP MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase truA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	tRNA pseudouridine synthase A HAMAP MF_00171		PRO_1000077082
Sites
	Active site	52	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9MCV8-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: E2A62312443C2BCD	FASTA	251	28,172
	10 20 30 40 50 60 MPRYKLTVEY DGTPYVGWQR QENGHAVQGA IEQAFKKFCG EDLTLSAAGR TDAGVHATAQ 70 80 90 100 110 120 VAHVDLAKDW GAGKVRDAVN AHLVMADERI SILNVEKTTD TFDARFSARA RHYLYRIHNR 130 140 150 160 170 180 RAPLAVDYQR AWWVQKQLDA DAMHEAAQRL LGEHDFTTFR ATQCQAKSPV KTLDRLDVTR 190 200 210 220 230 240 NGDMVEMRVS ARSFLHNQVR SFAGSLMEVG VGRWTADDLQ AALEARDRKA CGQVAPPYGL 250 YLVGVDYAFP F

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000873 Genomic DNA. Translation: ABX64195.1.
RefSeq	YP_001594966.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5787297.
GenomeReviews	Gene locus BCAN_B1053 in contig CP000873_GR.
KEGG	bcs:BCAN_B1053.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A9MCV8. NNDNTIQ.
Family and domain databases
HAMAP	MF_00171. [Tree]
InterPro	IPR001406. tRNA_psdUrid_synth. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_BRUC2

Accession

Primary (citable) accession number: A9MCV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	June 16, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)