Reviewed, UniProtKB/Swiss-Prot A9MCV1 (ARGB_BRUC2)

Names and origin

Protein names

Recommended name:
    Acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name=AGK
    N-acetyl-L-glutamate 5-phosphotransferase

Gene names

Name:	argB
Ordered Locus Names:	BCAN_B1046

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Acetylglutamate kinase HAMAP MF_00082		PRO_1000075303
Regions
	Region	67 – 68	2	Substrate binding By similarity
Sites
	Binding site	89	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Site	32	1	Transition state stabilizer By similarity
	Site	254	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9MCV1-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: B518ACFFB28AE924	FASTA	296	31,499
	10 20 30 40 50 60 MTTLENPEMQ AQLLSAALPY MQRYENKHVV VKYGGHAMGN PELGKAFARD VALLKQSGVN 70 80 90 100 110 120 PIVVHGGGPQ IQAMLTKLGI ESRFEGGLRV TDEKTVEVVE MVLAGSINKE IVALINAEGE 130 140 150 160 170 180 WAIGLCGKDG NMVFAQKAHK TVIDPDSNIE KVLDLGFVGE PAEVDRTLLD LLARSEMIPV 190 200 210 220 230 240 IAPVAPGRDG HTYNINADTF AGAIAGALAA TRLLFLTDVP GVLDKDKKLI KELSVADAQA 250 260 270 280 290 LIRDGTISGG MIPKVETCID AIRRGVEGVV ILNGKTPHSV LLELFTEHGA GTLIVP

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000873 Genomic DNA. Translation: ABX64188.1.
RefSeq	YP_001594959.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5787280.
GenomeReviews	Gene locus BCAN_B1046 in contig CP000873_GR.
KEGG	bcs:BCAN_B1046.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FVIKYGG.
Family and domain databases
HAMAP	MF_00082. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR011148. GlcNAc_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BRUC2

Accession

Primary (citable) accession number: A9MCV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)