A9MBD4 (BIOD_BRUC2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	BCAN_B0492

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_1000079269
Regions
	Nucleotide binding	100 – 103	4	ATP By similarity
	Nucleotide binding	184 – 186	3	ATP By similarity
Sites
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	100	1	Magnesium 2 By similarity
	Binding site	37	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9MBD4 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: F7D5083F8F33E1DC	FASTA	212	22,988
	10 20 30 40 50 60 MNSRLIVTGT DTGIGKTVFS AALCHALGAA YWKPVQSGLE EETDSEIVAR LAQASPQRIL 70 80 90 100 110 120 PEAWRLNTPA SPHLSARLDG VEIRPEEMHI PATSLPLVIE GAGGLLVPLN DKTLFADLFA 130 140 150 160 170 180 IWRIPAILCA RAALGTINHT LLSLEAMRSR DIPVLGVAFI GEANEDTETT IAHLGRVKRL 190 200 210 GRLPLLDDLS PEKLHHSFAR NFHIDDFAGV AR

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23365 / NCTC 10854.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000873 Genomic DNA. Translation: ABX63671.1.
RefSeq	YP_001594442.1. NC_010104.1.
3D structure databases
ProteinModelPortal	A9MBD4.
ModBase	Search...
Protein-protein interaction databases
STRING	A9MBD4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5786636.
GenomeReviews	Gene locus BCAN_B0492 in contig CP000873_GR.
KEGG	bcs:BCAN_B0492.
PATRIC	17834182. VBIBruCan25663_2713.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	WKPIQSG.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	BCAN483179:BCAN_B0492-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BRUC2

Accession

Primary (citable) accession number: A9MBD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program