Reviewed, UniProtKB/Swiss-Prot A9MB89 (GLPK_BRUC2)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	BCAN_B0445

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Glycerol kinase HAMAP MF_00186		PRO_1000077410
Regions
	Nucleotide binding	411 – 415	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	308	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9MB89-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: F7CF07660DB7ED8E	FASTA	498	54,737
	10 20 30 40 50 60 MSGYILAIDQ GTTSTRSMLF DRNMRVVGLG QQEFTQHFPS SGWVEHDAEE IWKSVQSTIR 70 80 90 100 110 120 IALAQAGISA ADVAAIGITN QRETTVVWDR ISGKPVHRAI VWQDRRTAQF CDELKRRNLE 130 140 150 160 170 180 PLFTEKTGLL LDPYFSGTKL AWLLNHVPGL RERAQKGQVC FGTIDSWLIY KLTGGKAHVT 190 200 210 220 230 240 DATNASRTLI YHIGENRWDD ELLDILGIPA AMLPEVKDCA ADFGMTDPAL FGVSIPILGV 250 260 270 280 290 300 AGDQQAAVIG NACFEPGMMK STYGTGCFAL LNTGTDRVTS SNRLLTTIAY RLDGVTTYAL 310 320 330 340 350 360 EGSIFIAGAA VQWLRDEMGF ISVASEVSAL AEKADPNQRI YLVPAFTGLG APYWDAEARG 370 380 390 400 410 420 AIFGLTRGTG RAEFARAALE SVAYQTFDLL EAMQGDWKGA TNHTVLRVDG GMVASDWTMQ 430 440 450 460 470 480 RLADILNAPV DRPVFLETTV LGAAWLAASR AGIWPDRKGF SERWQRDCRF EPAMPEKERE 490 SAIAGWRDSV SRCLTRPQ

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000873 Genomic DNA. Translation: ABX63626.1.
RefSeq	YP_001594397.1.
3D structure databases
SMR	A9MB89. Positions 4-494.
ModBase	Search...
Genome annotation databases
GeneID	5786480.
GenomeReviews	Gene locus BCAN_B0445 in contig CP000873_GR.
KEGG	bcs:BCAN_B0445.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	KFADNWR.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BRUC2

Accession

Primary (citable) accession number: A9MB89

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)