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Protein

Imidazole glycerol phosphate synthase subunit HisF

Gene

hisF

Organism
Brucella canis (strain ATCC 23365 / NCTC 10854)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.UniRule annotation

Catalytic activityi

5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O.UniRule annotation

Pathwayi: L-histidine biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase regulatory subunit (hisZ)
  2. Phosphoribosyl-ATP pyrophosphatase (hisE)
  3. Phosphoribosyl-AMP cyclohydrolase (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
  6. Imidazoleglycerol-phosphate dehydratase (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. no protein annotated in this organism
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei12UniRule annotation1
Active sitei131UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00031; UER00010.

Names & Taxonomyi

Protein namesi
Recommended name:
Imidazole glycerol phosphate synthase subunit HisFUniRule annotation (EC:4.1.3.-UniRule annotation)
Alternative name(s):
IGP synthase cyclase subunitUniRule annotation
IGP synthase subunit HisFUniRule annotation
ImGP synthase subunit HisFUniRule annotation
Short name:
IGPS subunit HisFUniRule annotation
Gene namesi
Name:hisFUniRule annotation
Ordered Locus Names:BCAN_A2131
OrganismiBrucella canis (strain ATCC 23365 / NCTC 10854)
Taxonomic identifieri483179 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000001385 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000840501 – 261Imidazole glycerol phosphate synthase subunit HisFAdd BLAST261

Interactioni

Subunit structurei

Heterodimer of HisH and HisF.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA9M9R9.
SMRiA9M9R9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HisA/HisF family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000224612.
KOiK02500.
OMAiKRIVPCL.

Family and domain databases

CDDicd04731. HisF. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01013. HisF. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR004651. HisF.
IPR011060. RibuloseP-bd_barrel.
PfamiView protein in Pfam
PF00977. His_biosynth. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00735. hisF. 1 hit.

Sequencei

Sequence statusi: Complete.

A9M9R9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLKARVIPC LDVKDGRVVK GVNFVDLIDA GDPVEAARAY DAAGADELCF
60 70 80 90 100
LDITASSDNR ETIFDVVART AEQCFMPLTV GGGVRQVADI RKLLLAGADK
110 120 130 140 150
VSINTAAVKN PEFVAEAADK FGNQCIVVAI DAKKVSGAGE NDRWEIFTHG
160 170 180 190 200
GRQPTGIDAV EFAQKVVDLG AGEILLTSMD RDGTKAGYDV ALTRAVADSV
210 220 230 240 250
RAPVIASGGV GTLDHLVAGI RDGHATAVLA ASIFHFGTYT IGEAKRYMAE
260
AGIPMRLDPV R
Length:261
Mass (Da):27,474
Last modified:February 5, 2008 - v1
Checksum:iDECA75E3993D1720
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000872 Genomic DNA. Translation: ABX63114.1.
RefSeqiWP_002965151.1. NC_010103.1.

Genome annotation databases

EnsemblBacteriaiABX63114; ABX63114; BCAN_A2131.
KEGGibcs:BCAN_A2131.

Similar proteinsi

Entry informationi

Entry nameiHIS6_BRUC2
AccessioniPrimary (citable) accession number: A9M9R9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: June 7, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families