A9M9Q4 (AROE_BRUC2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	BCAN_A2115

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000078114
Regions
	Nucleotide binding	134 – 138	5	NADP By similarity
Sites
	Active site	73	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	A9M9Q4 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: B0689B2E2675E43B	FASTA	289	30,408
	10 20 30 40 50 60 MDDKSMARGR KAFVTGFPIK HSRSPLIHGF WLKELGIDGS YEAVEVKPED FSSFAASLAA 70 80 90 100 110 120 NGFAGGNVTI PHKEAAYAAA ESLDEAACAI GAVNTLWLEN GRLCGGNTDA YGFAANLDAS 130 140 150 160 170 180 APGWDKADRA LVLGAGGASR AVVHALLSRG VCHVSVVNRT LSRAEELAAH FGARVYAHGW 190 200 210 220 230 240 DEAQALVSNA GLIVNTTALG MSGHGEGQDF PIDLTCAPKE AVATDIVYVP LRTAFLNKAE 250 260 270 280 KAGLKTVDGL GMLLHQAVPG FERWFGQRPQ VTQALREHIL ADMAKAGAL

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23365 / NCTC 10854.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000872 Genomic DNA. Translation: ABX63099.1.
RefSeq	YP_001593870.1. NC_010103.1.
3D structure databases
ProteinModelPortal	A9M9Q4.
ModBase	Search...
Protein-protein interaction databases
STRING	A9M9Q4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5784324.
GenomeReviews	Gene locus BCAN_A2115 in contig CP000872_GR.
KEGG	bcs:BCAN_A2115.
PATRIC	17832954. VBIBruCan25663_2103.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553408.
OMA	LHLKTHG.
ProtClustDB	PRK00258.
Enzyme and pathway databases
BioCyc	BCAN483179:BCAN_A2115-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_BRUC2

Accession

Primary (citable) accession number: A9M9Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program