Reviewed, UniProtKB/Swiss-Prot A9M9H8 (BETA_BRUC2)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	BCAN_A0565

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Choline dehydrogenase		PRO_1000083492
Regions
	Nucleotide binding	4 – 33	30	FAD Probable
Sites
	Active site	465	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9M9H8-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: BE54097F5EFBEA02	FASTA	549	60,594
	10 20 30 40 50 60 MEADFVIIGS GSAGSAMAYR LSEDGRYSVI VIEYGVPDVG PLIQMPAALS FPMNMETYDW 70 80 90 100 110 120 GFSSEPEPHI GGRSLVTPRG KVLGGSSSIN GMVYVRGHAC DFDHWSQSGA RGWAYADVLP 130 140 150 160 170 180 YFKRMENSQG GQEGWRGTNG PLYVQRGKRD NPLFHAFVEA GHQAGFEVAD DYNGEKQEGF 190 200 210 220 230 240 GPMEQTIHNG RRWSAANAYL KPALKRPNVK LVKGFARKIV LEGKRAVGVE IEAGRTFSTI 250 260 270 280 290 300 RARREVIIAA SSINSPKLLM LSGIGPAAHL KEHGIDLVAD RPGVGQNLQD HLEVYIQQEC 310 320 330 340 350 360 TQPITLYSEL NLFSKARIGV EWLLFKTGDG ATNHFESAAF VRSKAGVEYP DIQYHFLPVA 370 380 390 400 410 420 IRYDGKAAAQ SHGFQAHVGP MRSKSRGSVT LRSANPREKP VIKFNYMSHE DDWADFRHCV 430 440 450 460 470 480 RLTREIFGQA AFDPYRGAEI QPGAHVQTDD EIDNFIREHV ESAFHPCGTC KMGAVDDPMA 490 500 510 520 530 540 VVDPECRVIG VEGLRVADSS IFPRITNGNL NGPSIMVGEK ASDHILGRTP LARSNQEPWI NPRWQVSDR

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000872 Genomic DNA. Translation: ABX61642.1.
RefSeq	YP_001592413.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5785572.
GenomeReviews	Gene locus BCAN_A0565 in contig CP000872_GR.
KEGG	bcs:BCAN_A0565.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_BRUC2

Accession

Primary (citable) accession number: A9M9H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	November 25, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)