Reviewed, UniProtKB/Swiss-Prot A9M9E9 (DCUP_BRUC2)

Names and origin

Protein names

Recommended name:
    Uroporphyrinogen decarboxylase
      Short name=URO-D
      Short name=UPD
    EC=4.1.1.37

Gene names

Name:	hemE
Ordered Locus Names:	BCAN_A2112

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III By similarity.
Catalytic activity	Uroporphyrinogen III = coproporphyrinogen + 4 CO2. HAMAP MF_00218
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4. HAMAP MF_00218
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the uroporphyrinogen decarboxylase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	uroporphyrinogen decarboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Uroporphyrinogen decarboxylase HAMAP MF_00218		PRO_1000078066
Regions
	Region	23 – 27	5	Substrate binding By similarity
Sites
	Binding site	73	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	203	1	Substrate By similarity
	Binding site	318	1	Substrate By similarity
	Site	73	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9M9E9-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: DA4873C2CA945A82	FASTA	341	37,831
	10 20 30 40 50 60 MNRKVLKVID GETVFPPPIW MMRQAGRYLP EYRETRKKAG SFLDLCYSPD LAVEVTLQPI 70 80 90 100 110 120 RRFGFDAAIL FSDILVVPHA LGRDLRFEEG KGPLMTPIDA DEIFWLETEG VAKRLEPVYE 130 140 150 160 170 180 TVRLVREQLP DETTLLGFCG APWTVATYMI AGHGTPDQAP ARLFAYRFPE AFEKLLNDLA 190 200 210 220 230 240 DVSAEYLIEQ LGAGADAVQI FDSWSGVLDE DCFERFCIRP VARIVQKVRA VYPQARIIGF 250 260 270 280 290 300 PKGAGMLYAG YREKTGVDML GLDWSVPLSF AALLQEEGAV QGNLDPLRVV AGGNALDEGV 310 320 330 340 DAILERMGQG PLVFNLGHGI TPQAPIENVQ RMIDRVRGGK S

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000872 Genomic DNA. Translation: ABX63096.1.
RefSeq	YP_001593867.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5785785.
GenomeReviews	Gene locus BCAN_A2112 in contig CP000872_GR.
KEGG	bcs:BCAN_A2112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VFTKGGG.
Family and domain databases
HAMAP	MF_00218. [Tree]
InterPro	IPR006361. Uroporphyrinogen_deCO2ase_HemE. IPR000257. Uroporphyrinogen_deCOase. [Graphical view]
PANTHER	PTHR21091:SF2. HemE. 1 hit.
Pfam	PF01208. URO-D. 1 hit. [Graphical view]
ProDom	PD003225. Uro_decarbxyls. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01464. hemE. 1 hit.
PROSITE	PS00906. UROD_1. 1 hit. PS00907. UROD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCUP_BRUC2

Accession

Primary (citable) accession number: A9M9E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)