Reviewed, UniProtKB/Swiss-Prot A9M8M3 (GCP_BRUC2)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BCAN_A1931

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc By similarity. HAMAP MF_01445
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_1000087468
Sites
	Metal binding	115	1	Zinc Potential
	Metal binding	119	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A9M8M3-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 9F005EAD34760BC4	FASTA	359	37,982
	10 20 30 40 50 60 MRVLGIETSC DETAAAIVQR DDMGEGRILS NVVLSQIAEH EPYGGVVPEI AARAHVEALD 70 80 90 100 110 120 RLVDRALNDA GLKLYEVDAV AATAGPGLIG GLIVGLMTAK ALAMAAQKPF YAVNHLEGHA 130 140 150 160 170 180 LTARLTDGLP FPYLLLLVSG GHTQMVLVRG IGDYERLGTT IDDALGEAFD KTAKLLGLPY 190 200 210 220 230 240 PGGPAVERMA LQGDQKRFAL PRPLKGEARL DFSFSGLKTA VRQTATELVP LTDQDVTDIC 250 260 270 280 290 300 ASFQAAVADT LSDRVGRSLE RFKTEFPDCA TPSLVVAGGV AANKTLRAAL ENLCTRHGFA 310 320 330 340 350 FIAPPLNLCT DNAAMIAWAG AERAATQAPD SLDIAPRSRW PLDEKSAPVF GTGRRGAKA

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000872 Genomic DNA. Translation: ABX62921.1.
RefSeq	YP_001593692.1.
3D structure databases
SMR	A9M8M3. Positions 1-348.
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	5785342.
GenomeReviews	Gene locus BCAN_A1931 in contig CP000872_GR.
KEGG	bcs:BCAN_A1931.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304663.
OMA	CKRALKQ.
Family and domain databases
HAMAP	MF_01445. Glycoptase_bact. [Tree]
InterPro	IPR009180. Pept_M22_O-sialoglycoprot. IPR000905. Peptidase_M22. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BRUC2

Accession

Primary (citable) accession number: A9M8M3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)