Reviewed, UniProtKB/Swiss-Prot A9M880 (PROA_BRUC2)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	BCAN_A1881

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000080476

Sequences

	Sequence		Length	Mass (Da)
	A9M880-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: E857329C6DF1B9E7	FASTA	427	44,757
	10 20 30 40 50 60 MLVKADMTKD IAQVMAEVGR KAKAAAAPLS IATSEQKNKA LNAAADAILE ARADILEANR 70 80 90 100 110 120 LDLANAEKNG MAASFVDRLT LNEARIDAIA EDIRAIATLP DPVGEVIAEW DRPNGLHIER 130 140 150 160 170 180 VRTPLGVIGV IYESRPNVTA DAGALCLKAG NAVILRGGSD SAHSSAAIHK ALVKGLEAAN 190 200 210 220 230 240 LPADAIQIVP VTDRAAVGEM LKGLGGAIDV IVPRGGKSLV ARVQSEARVP VFAHLEGICH 250 260 270 280 290 300 LYIDKSADLD MARRIALDAK MRRTGICGAA ETLLVDRAVA STHLAPILGD LAAGGCEIRG 310 320 330 340 350 360 SAEVLALYPA AKPATEEDWS TEYLDAIISV ALVDGISGAI DHINRYSSHH TEAIVAEDAQ 370 380 390 400 410 420 TVARFFNEID SAILLHNAST QFADGGEFGM GAEIGIATGK MHARGPVGVE QLTSFKYRVR GSGQVRG

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000872 Genomic DNA. Translation: ABX62878.1.
RefSeq	YP_001593649.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5784302.
GenomeReviews	Gene locus BCAN_A1881 in contig CP000872_GR.
KEGG	bcs:BCAN_A1881.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A9M880. APIISIK.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BRUC2

Accession

Primary (citable) accession number: A9M880

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)