Reviewed, UniProtKB/Swiss-Prot A9M849 (ACSA_BRUC2)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase
    Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	BCAN_A1849

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000084999
Sites
	Active site	517	1	By similarity
Amino acid modifications
	Modified residue	611	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9M849-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 7FB222A3EE6476CE	FASTA	651	72,731
	10 20 30 40 50 60 MSEKLYPVLP EAKKNTLIDN ETYLEWYEES VSDPDGFWAK HGRRIDWFKP FTKVKNTDFN 70 80 90 100 110 120 GDVTIKWYED GVTNVSYNCI DRHLKSRGDK VAIIWEGDNP YIDKKITYRE LYENVCRMAN 130 140 150 160 170 180 VLKKHGVKKG DRVTIYLPMI PEAAYAMLAC ARIGAVHSVV FAGFSPEALA GRIVDCESTF 190 200 210 220 230 240 VITADEGVRG GKPVALKENT DTAIDIAAKQ YVMVNKVLVV RRTGGKVSWG RGRDLWYHQE 250 260 270 280 290 300 VASVEPHCEP EPMNAEDPLF ILYTSGSTGK PKGVLHTTGG YLVYASMTHQ YVFDYHDGEI 310 320 330 340 350 360 YWCTADVGWV TGHSYIVYGP LANGATTLMF EGVPNFPDQG RFWEVVDKHH VNIFYTAPTA 370 380 390 400 410 420 LRALMGAGDE FVTRSSRSTL RLLGSVGEPI NPEAWEWYYN VVGDQKCPIV DTWWQTENGG 430 440 450 460 470 480 ILITPLPGAT DLKPGSATRP FFGVKPVLVD NEGNVQEGVA DGNLCISDSW PGQMRTVYGD 490 500 510 520 530 540 HKRFIETYFS TYKGMYFSGD GCRRDEDGYY WITGRVDDVL NISGHRLGTA EIESALVSHH 550 560 570 580 590 600 SVSEAAVVGY PHPIKGQGIY CYVTLMTGAD AQDPDELRKE LVQHVRKEIG PIATPDKIQF 610 620 630 640 650 APGLPKTRSG KIMRRILRKI AEDEFGALGD TSTLADPGVV DDLIENRQNK K

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000872 Genomic DNA. Translation: ABX62847.1.
RefSeq	YP_001593618.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5785190.
GenomeReviews	Gene locus BCAN_A1849 in contig CP000872_GR.
KEGG	bcs:BCAN_A1849.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ETASEHC.
Family and domain databases
HAMAP	MF_01123. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_BRUC2

Accession

Primary (citable) accession number: A9M849

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)