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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Brucella canis (strain ATCC 23365 / NCTC 10854)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi25 – 306ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCAN483179:GJ7I-1714-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:BCAN_A1732
OrganismiBrucella canis (strain ATCC 23365 / NCTC 10854)
Taxonomic identifieri483179 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000001385 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448tRNA(Ile)-lysidine synthasePRO_1000085363Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA9M7J1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000236508.
KOiK04075.
OMAiCTHNASA.
OrthoDBiEOG6NKR0V.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

A9M7J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSPVNIFK PFGLGRAKAV IAAVSGGSDS LGLLFLLKDY LSTLESPPVL
60 70 80 90 100
IAVTVDHKLR AESALEAENV GLLCQKHGIM HCVLSWDDPK PAHGLAAAAR
110 120 130 140 150
TARYRLLVQA ARDAGAGFIV TGHTENDQIE TFLMRKARSG HCEARGLAAM
160 170 180 190 200
SPRSLLEGSV ELARPLLTVS RQALRDELTR RGIAWVDDPS NANIDYERPR
210 220 230 240 250
VRLGVAAEAD GQEVLEQIAQ AGAARERDNA ALVEALADPA TLGVDAAGMM
260 270 280 290 300
FLNADCYAAL SPGARQLFSG LLASIAGGRR FLPGDGERRR IERMLSGQDA
310 320 330 340 350
PRRLTVFGAL IERGEKGAPH RFRRERRNLP KLDLVPGQHI VWDGRFCFFN
360 370 380 390 400
SGGRSFEIAP PGRQELIDFL KNSGRDIESR RCEALLISPA LYEGGKLASV
410 420 430 440
PFLPGAEWPQ GVHIERHFAI FDHVLPGHDF ALAQAVEARL GRACAEIS
Length:448
Mass (Da):48,546
Last modified:February 5, 2008 - v1
Checksum:i395015CEA7C30899
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000872 Genomic DNA. Translation: ABX62738.1.
RefSeqiWP_004692771.1. NC_010103.1.

Genome annotation databases

EnsemblBacteriaiABX62738; ABX62738; BCAN_A1732.
KEGGibcs:BCAN_A1732.
PATRICi17832164. VBIBruCan25663_1720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000872 Genomic DNA. Translation: ABX62738.1.
RefSeqiWP_004692771.1. NC_010103.1.

3D structure databases

ProteinModelPortaliA9M7J1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX62738; ABX62738; BCAN_A1732.
KEGGibcs:BCAN_A1732.
PATRICi17832164. VBIBruCan25663_1720.

Phylogenomic databases

HOGENOMiHOG000236508.
KOiK04075.
OMAiCTHNASA.
OrthoDBiEOG6NKR0V.

Enzyme and pathway databases

BioCyciBCAN483179:GJ7I-1714-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23365 / NCTC 10854.

Entry informationi

Entry nameiTILS_BRUC2
AccessioniPrimary (citable) accession number: A9M7J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: November 11, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.