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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Brucella canis (strain ATCC 23365 / NCTC 10854)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 30ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processtRNA processing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:BCAN_A1732
OrganismiBrucella canis (strain ATCC 23365 / NCTC 10854)
Taxonomic identifieri483179 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000001385 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000853631 – 448tRNA(Ile)-lysidine synthaseAdd BLAST448

Proteomic databases

PRIDEiA9M7J1

Structurei

3D structure databases

ProteinModelPortaliA9M7J1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000236508
KOiK04075
OMAiNWYNNIQ

Family and domain databases

CDDicd01992 PP-ATPase, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_01161 tRNA_Ile_lys_synt, 1 hit
InterProiView protein in InterPro
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR012094 tRNA_Ile_lys_synt
IPR012795 tRNA_Ile_lys_synt_N
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
TIGRFAMsiTIGR02432 lysidine_TilS_N, 1 hit

Sequencei

Sequence statusi: Complete.

A9M7J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSPVNIFK PFGLGRAKAV IAAVSGGSDS LGLLFLLKDY LSTLESPPVL
60 70 80 90 100
IAVTVDHKLR AESALEAENV GLLCQKHGIM HCVLSWDDPK PAHGLAAAAR
110 120 130 140 150
TARYRLLVQA ARDAGAGFIV TGHTENDQIE TFLMRKARSG HCEARGLAAM
160 170 180 190 200
SPRSLLEGSV ELARPLLTVS RQALRDELTR RGIAWVDDPS NANIDYERPR
210 220 230 240 250
VRLGVAAEAD GQEVLEQIAQ AGAARERDNA ALVEALADPA TLGVDAAGMM
260 270 280 290 300
FLNADCYAAL SPGARQLFSG LLASIAGGRR FLPGDGERRR IERMLSGQDA
310 320 330 340 350
PRRLTVFGAL IERGEKGAPH RFRRERRNLP KLDLVPGQHI VWDGRFCFFN
360 370 380 390 400
SGGRSFEIAP PGRQELIDFL KNSGRDIESR RCEALLISPA LYEGGKLASV
410 420 430 440
PFLPGAEWPQ GVHIERHFAI FDHVLPGHDF ALAQAVEARL GRACAEIS
Length:448
Mass (Da):48,546
Last modified:February 5, 2008 - v1
Checksum:i395015CEA7C30899
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000872 Genomic DNA Translation: ABX62738.1
RefSeqiWP_004692771.1, NC_010103.1

Genome annotation databases

EnsemblBacteriaiABX62738; ABX62738; BCAN_A1732
KEGGibcs:BCAN_A1732

Similar proteinsi

Entry informationi

Entry nameiTILS_BRUC2
AccessioniPrimary (citable) accession number: A9M7J1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: May 23, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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