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A9M752

- MASZ_BRUC2

UniProt

A9M752 - MASZ_BRUC2

Protein

Malate synthase G

Gene

glcB

Organism
Brucella canis (strain ATCC 23365 / NCTC 10854)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei123 – 1231Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei281 – 2811Acetyl-CoAUniRule annotation
    Binding sitei318 – 3181Acetyl-CoAUniRule annotation
    Active sitei345 – 3451Proton acceptorUniRule annotation
    Binding sitei345 – 3451GlyoxylateUniRule annotation
    Metal bindingi437 – 4371MagnesiumUniRule annotation
    Binding sitei437 – 4371GlyoxylateUniRule annotation
    Metal bindingi465 – 4651MagnesiumUniRule annotation
    Binding sitei546 – 5461Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei636 – 6361Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBCAN483179:GJ7I-1673-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:BCAN_A1689
    OrganismiBrucella canis (strain ATCC 23365 / NCTC 10854)
    Taxonomic identifieri483179 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000001385: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 728728Malate synthase GPRO_1000082698Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei622 – 6221Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi483179.BCAN_A1689.

    Structurei

    3D structure databases

    ProteinModelPortaliA9M752.
    SMRiA9M752. Positions 10-725.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni130 – 1312Acetyl-CoA bindingUniRule annotation
    Regioni462 – 4654Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A9M752-1 [UniParc]FASTAAdd to Basket

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    MGSAEKRNYV EIEGLAVAPE LVEFLAKEAA PGTGVEPEKF WKGFAAIIRD    50
    LAPKNRALLA KRDELQARID AWYKENRDKG YSQADYQQFL KDIGYLLPEG 100
    GAFSVSTTNV DPEITHIAGP QLVVPVMNAR YALNAANARW GSLYDALYGT 150
    DAISEADGAE KGKGYNPKRG EKVIAWAKNF LDESAPLSTG KWADVAGLAV 200
    NDGKLEIRLT DGSATTLKDE SQFKGYNGDA ASPTNVLLAK HNMHVDIVIN 250
    ADHPIGKTDP AHIADVVLES AISTIQDCED SIAAVDAEDK VAVYRNWLRL 300
    MNGKLEDTFE KNGKQMTRRL NGDRTYTAPD GSTLTLKGRS LMLVRNVGHL 350
    MTNPAILDAE GNEVPEGIMD AAFTSLIALH DIGPNGRHMN SREGSVYIVK 400
    PKMHGPEEVA FANEIFTRTE EMLGMKPNTL KIGIMDEERR TTVNLKEAIR 450
    AAKDRVVFIN TGFLDRTGDE IHTSMEAGPM IRKGDMKQAA WIGAYEQWNV 500
    DIGLECGLSG HAQIGKGMWA MPDMMAAMLE QKIAHPKAGA NTAWVPSPTA 550
    ATLHATHYHK IDVAAVQEKL KSRPRAKLDD ILSVPVAVRP NWTPDDIQHE 600
    IDNNAQGILG YVVRWIDQGV GCSKVPDINN VGLMEDRATL RISAQHIANW 650
    LYHGVVSEAQ VMETMKRMAA IVDKQNEGDP LYRPMAADFD KSIAFQAACD 700
    LVFKGREQPN GYTEPVLHRR RLELKQAS 728
    Length:728
    Mass (Da):80,085
    Last modified:February 5, 2008 - v1
    Checksum:i56EF98BD06D7970F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000872 Genomic DNA. Translation: ABX62699.1.
    RefSeqiYP_001593470.1. NC_010103.1.

    Genome annotation databases

    EnsemblBacteriaiABX62699; ABX62699; BCAN_A1689.
    GeneIDi5785193.
    KEGGibcs:BCAN_A1689.
    PATRICi17832078. VBIBruCan25663_1678.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000872 Genomic DNA. Translation: ABX62699.1 .
    RefSeqi YP_001593470.1. NC_010103.1.

    3D structure databases

    ProteinModelPortali A9M752.
    SMRi A9M752. Positions 10-725.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 483179.BCAN_A1689.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABX62699 ; ABX62699 ; BCAN_A1689 .
    GeneIDi 5785193.
    KEGGi bcs:BCAN_A1689.
    PATRICi 17832078. VBIBruCan25663_1678.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci BCAN483179:GJ7I-1673-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 23365 / NCTC 10854.

    Entry informationi

    Entry nameiMASZ_BRUC2
    AccessioniPrimary (citable) accession number: A9M752
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3