A9M6N0 (AROA_BRUC2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BCAN_A0026

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000077981

Sequences

	Sequence		Length	Mass (Da)
	A9M6N0 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: E571FAB83C049FDC	FASTA	450	47,249
	10 20 30 40 50 60 MSHSACPKPA TARHSQALTG EIRIPGDKSI SHRSFMFGGL ASGKTRITGL LEGEDVINTG 70 80 90 100 110 120 RAMQAMGARI RKEGDVWIIN GVGNGCLLQP EAPLDFGNAG TGARLTMGLV GTYDMKTSFI 130 140 150 160 170 180 GDASLSKRPM GRVLNPLREM GVQVEAAEGD RMPLTLIGPR TANPIAYRVP MASAQVKSAV 190 200 210 220 230 240 LLAGLNTPGV TTVIEPVMTR DHTEKMLQGF GADLTVETDK DGVRHIRIVG QGKLTGQTID 250 260 270 280 290 300 VPGDPSSTAF PLVAALLVEG SDVTIRNVLM NPTRTGLILT LQEMGADIEI IDPRLAGGED 310 320 330 340 350 360 VADLRVRASK LKGVVVPPER APSMIDEYPV LAIAASFAEG ETVMDGLDEL RVKESDRLAA 370 380 390 400 410 420 VARGLEANGV DCTEGEMSLT VRGRPGGKGL GGGTVATHLD HRIAMSFLVM GLASEKPVTV 430 440 450 DDSTMIATSF PEFMGMMAGL GAKIAESGAE

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23365 / NCTC 10854.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000872 Genomic DNA. Translation: ABX61131.1.
RefSeq	YP_001591902.1. NC_010103.1.
3D structure databases
ProteinModelPortal	A9M6N0.
SMR	A9M6N0. Positions 8-447.
ModBase	Search...
Protein-protein interaction databases
STRING	A9M6N0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5785816.
GenomeReviews	Gene locus BCAN_A0026 in contig CP000872_GR.
KEGG	bcs:BCAN_A0026.
PATRIC	17828699. VBIBruCan25663_0028.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	LECANIS.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	BCAN483179:BCAN_A0026-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BRUC2

Accession

Primary (citable) accession number: A9M6N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program