Reviewed, UniProtKB/Swiss-Prot A9M693 (MRAY_BRUC2)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	BCAN_A1467

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity. HAMAP MF_00038
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00038.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000074534
Regions
	Transmembrane	27 – 47	21	Potential
	Transmembrane	71 – 91	21	Potential
	Transmembrane	93 – 113	21	Potential
	Transmembrane	128 – 148	21	Potential
	Transmembrane	168 – 188	21	Potential
	Transmembrane	199 – 219	21	Potential
	Transmembrane	239 – 259	21	Potential
	Transmembrane	262 – 282	21	Potential
	Transmembrane	288 – 308	21	Potential
	Transmembrane	337 – 357	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	A9M693-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 72C41260FD120E78	FASTA	360	38,745
	10 20 30 40 50 60 MLMFLTHFAE HVTPFNVFRY ITFRTGGAMI TSALIVFLFG PTIINSLRVR QGKGQPIRAD 70 80 90 100 110 120 GPQTHFKKAG TPTMGGLMIM TGILASCLLW ANLASVYVWV VLMVSVGFGA IGFYDDYLKV 130 140 150 160 170 180 TKQSDKGFSG KARLGIEFLI AAIAAFTIMR AGQEPFSSSL TFPFVKQLVI NLSWFFIPFA 190 200 210 220 230 240 AFVMVGAGNA VNLTDGLDGL AIVPVMVAAA SFGFIAYLSG NAIFADYLQI HFVPGTGELA 250 260 270 280 290 300 VVLGAVIGAG LGFLWFNAPP AAIFMGDTGS LALGGMLGTV AVATKHEIVL AIIGGLFVME 310 320 330 340 350 360 ALSVIIQVGF FKMTGRRVFL MAPIHHHFEK KGWTESQVVI RFWIVAIILA MIGLSTLKLR

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000872 Genomic DNA. Translation: ABX62498.1.
RefSeq	YP_001593269.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5786036.
GenomeReviews	Gene locus BCAN_A1467 in contig CP000872_GR.
KEGG	bcs:BCAN_A1467.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG708263.
OMA	HHHFEQK.
Family and domain databases
HAMAP	MF_00038. MraY. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_BRUC2

Accession

Primary (citable) accession number: A9M693

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)