Reviewed, UniProtKB/Swiss-Prot A9M679 (DNLJ_BRUC2)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.2
Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Ordered Locus Names:	BCAN_A1453

Organism

Brucella canis (strain ATCC 23365 / NCTC 10854) [Complete proteome] [HAMAP]

Taxonomic identifier

483179 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588
Catalytic activity	NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588
Cofactor	Magnesium or manganese By similarity. HAMAP MF_01588
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (NAD+) activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	DNA ligase HAMAP MF_01588		PRO_0000340330
Regions
	Domain	640 – 719	80	BRCT
	Nucleotide binding	42 – 46	5	NAD By similarity
	Nucleotide binding	92 – 93	2	NAD By similarity
Sites
	Active site	128	1	N6-AMP-lysine intermediate By similarity
	Metal binding	430	1	Zinc By similarity
	Metal binding	433	1	Zinc By similarity
	Metal binding	448	1	Zinc By similarity
	Metal binding	454	1	Zinc By similarity
	Binding site	126	1	NAD By similarity
	Binding site	149	1	NAD By similarity
	Binding site	185	1	NAD By similarity
	Binding site	301	1	NAD By similarity
	Binding site	325	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9M679-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 122949D2FB8B1B79	FASTA	719	78,681
	10 20 30 40 50 60 MSDISVEKLT ELEAAAELER LARAIAHHDE LYHAKDRPEI SDAAYDALKR RNEAIEAHFP 70 80 90 100 110 120 ALVRDDSPSR RVGAAPALAT FAPVVHARPM LSLDNAFSDE DVRDFVGSVY RFLGQLPDDS 130 140 150 160 170 180 IAFTAEPKID GLSMSIRYEN GILVSGATRG DGTTGENVTA NIRTIAEIPN RLPAGAPAVV 190 200 210 220 230 240 EVRGEVYMAK SDFLTLNAQM EAEGKQTYVN PRNTAAGSLR QLDAKVTASR KLRFFAYAWG 250 260 270 280 290 300 EMSDMPADTQ LGMVEVFRQW GFPVNPLMKR FNSVDGLLAH YRAIGMERPT LDYDIDGVVY 310 320 330 340 350 360 KVDRLDLQTR LGFRSRSPRW AIAHKFPAEQ ALTILRGIDI QVGRTGALTP VARLEPITVG 370 380 390 400 410 420 GVVVTNATLH NEDYIKGIGQ KGEPIREGRD IRIGDSVIVQ RAGDVIPQIV DVVLEEGKKR 430 440 450 460 470 480 GEPYQFPHVC PACGSHAVRE EGEAVRRCTG GLICPAQAVE RIRHFVSRNA FDIEGLGEKQ 490 500 510 520 530 540 VEFFFNAEDP ALCIRSPADI FTLKKRQENS LTKLQNIEGF GATSVKKLYD AIDARREIAL 550 560 570 580 590 600 HRFLFGLGIR HVGEVNAKRL ARAYLSYAAF EKAALEAVPP KEGDRTDKGS EAWQDMLAVE 610 620 630 640 650 660 GIGSIVAEAV VDFYGEPHNR EVLAALLAEV TPLDEEARVA TGSPVEGKTV VFTGSLERMS 670 680 690 700 710 RDEAKAMAER HGAKTAGSVS KKTDLVVAGP GAGSKLAKAT ELGIEVINED DWFKLVGED

References

[1]

"Brucella canis ATCC 23365 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000872 Genomic DNA. Translation: ABX62484.1.
RefSeq	YP_001593255.1.
3D structure databases
SMR	A9M679. Positions 14-629, 643-718.
ModBase	Search...
Genome annotation databases
GeneID	5784494.
GenomeReviews	Gene locus BCAN_A1453 in contig CP000872_GR.
KEGG	bcs:BCAN_A1453.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG620317.
OMA	IKHFASR.
Family and domain databases
HAMAP	MF_01588. DNA_ligase_A. [Tree]
InterPro	IPR001357. BRCT. IPR018239. DNA_ligase_AS. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR010994. RuvA_2-like. IPR004149. Znf_DNAligase_C4. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. PF03119. DNA_ligase_ZBD. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
SMART	SM00292. BRCT. 1 hit. SM00278. HhH1. 2 hits. SM00532. LIGANc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00575. dnlj. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLJ_BRUC2

Accession

Primary (citable) accession number: A9M679

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	February 5, 2008
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)