A9M4E3 (METE_NEIM0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	NMCC_0887

Organism

Neisseria meningitidis serogroup C (strain 053442) [Complete proteome] [HAMAP]

Taxonomic identifier

374833 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000077115
Sites
	Metal binding	643	1	Zinc By similarity
	Metal binding	645	1	Zinc By similarity
	Metal binding	728	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9M4E3 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 246A7AD2A8503095	FASTA	758	85,085
	10 20 30 40 50 60 MTTLHFSGFP RVGAFRELKF AQEKYWRKEI SEQELLAVAK DLREKNWKHQ AAANADYVAV 70 80 90 100 110 120 GDFTFYDHIL DLQVATGAIP ARFGFDSQNL SLEQFFQLAR GNKDQFAIEM TKWFDTNYHY 130 140 150 160 170 180 LVPEFHADTE FKANAKHYVQ QLQEAQALGL KAKPTVVGPL TFLWVGKEKG AVEFDRLSLL 190 200 210 220 230 240 PKLLPVYVEI LTALVEAGAE WIQIDEPALT VDLPKEWVEA YKDVYATLSK VSAKILLGTY 250 260 270 280 290 300 FGSVAEHAAL LKALPVDGLH IDLVRAPEQP DAFADYDKVL SVGVIDGRNI WRANLNKVLE 310 320 330 340 350 360 TVEPLQAKLG ERLWISSSCS LLHTPFDLSV EEKLKANKPD LYSWLAFTLQ KTQELRVLKA 370 380 390 400 410 420 ALNEGRDSVA EELAASQAAA DSRANSSEIH RADVAKRLAD LPANADQRKS PFVNRIKAQQ 430 440 450 460 470 480 AWLNLPLLPT TNIGSFPQTT EIRQARAAFK KGELSAADYE AAMKKEIALV VEEQEKLDLD 490 500 510 520 530 540 VLVHGEAERN DMVEYFGELL SGFAFTQYGW VQSYGSRCVK PPIIFGDVSR PEAMTVAWST 550 560 570 580 590 600 YAQSLTKRPM KGMLTGPVTI LQWSFVRNDI PRATVCKQIA LALNDEVLDL EKAGIKVIQI 610 620 630 640 650 660 DEPAIREGLP LKRADWDAYL NWAGESFRLS SAGCEDSTQI HTHMCYSEFN DILPAIAAMD 670 680 690 700 710 720 ADVITIETSR SDMELLTAFG KFKYPNDIGP GVYDIHSPRV PTEAEVEHLL RKAIEVVPVE 730 740 750 RLWVNPDCGL KTRGWKETLE QLQVMMNVTR KLRAELAK

References

[1]

"Characterization of ST-4821 complex, a unique Neisseria meningitidis clone." Peng J., Yang L., Yang F., Yang J., Yan Y., Nie H., Zhang X., Xiong Z., Jiang Y., Cheng F., Xu X., Chen S., Sun L., Li W., Shen Y., Shao Z., Liang X., Xu J., Jin Q. Genomics 91:78-87(2008) [PubMed: 18031983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 053442.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000381 Genomic DNA. Translation: ABX73069.1.
RefSeq	YP_001599025.1. NC_010120.1.
3D structure databases
ProteinModelPortal	A9M4E3.
ModBase	Search...
Protein-protein interaction databases
STRING	A9M4E3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000011928; EBNEIP00000011470; EBNEIG00000011928.
GeneID	5795486.
GenomeReviews	Gene locus NMCC_0887 in contig CP000381_GR.
KEGG	nmn:NMCC_0887.
PATRIC	20346309. VBINeiMen117761_1072.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000021045.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	NMEN374833:NMCC_0887-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_NEIM0

Accession

Primary (citable) accession number: A9M4E3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program