A9M455 (A9M455_NEIM0) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL ABX74149.1
Ordered Locus Names:	NMCC_2030

Organism

Neisseria meningitidis serogroup C (strain 053442) [Complete proteome] [HAMAP]

Taxonomic identifier

374833 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A9M455 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 3894D9A6FE0AF6B3	FASTA	748	84,737
	10 20 30 40 50 60 MGGDFEILAS YGHVRDLVPK SGAVDPDNGF AMKYQLISRN GKHVDAIVAG AKEAENIYLA 70 80 90 100 110 120 TDPDREGEAI SWHLLEILKS KRGLKNIKPQ RVVFHEITKN AVLDAVAHPR EIEMDLVDAQ 130 140 150 160 170 180 QARRALDYLV GFNLSPLLWK KIRRGLSAGR VQSPALRLIC ERENEIRAFE AQEYWTVHLD 190 200 210 220 230 240 SHKGRSKFTA KLAQYNGAKL EQFDLPNEAA QADVLKELEG KEAVVTAIEK KKRSRNPAAP 250 260 270 280 290 300 FTTSTMQQDA VRKLGFTTDR TMRTAQQLYE GIDVGQGAIG LITYMRTDSV NLADEALTEI 310 320 330 340 350 360 RHYIENKIGK EYLPSAAKQY KTKSKNAQEA HEAIRPTSVY RTPESVKPFL SADQFKLYQM 370 380 390 400 410 420 IWQRTVACQM TPAKFDQTTV DITVGKGVFR VTGQVQTFAG FLSVYEESSD DEEGEDSKKL 430 440 450 460 470 480 PEMSEGDKLP VDKLYGEQHF TTPPPRYNEA TLVKALEEYG IGRPSTYASI ISTLKDREYV 490 500 510 520 530 540 TLEQKRFMPT DTGDIVNKFL TEHFAQYVDY HFTAKLEDQL DEIAGGKRQW IPLMDKFWKP 550 560 570 580 590 600 FIKQVEEKEG IERAKFTTQE LDETCPKCGE HKLQIKFGKM GRFVACAGYP ECSYTRNVNE 610 620 630 640 650 660 TAEEAAERIA KAEAEQAELD GRECPKCGGR LVYKYSRTGS KFIGCANYPK CKHVEPLEKP 670 680 690 700 710 720 KDTGVQCPQC KKGNLVERKS RYGKLFYSCS TYPDCNYATW NPPVAEECPN CHWPVLTIKT 730 740 TKRWGVEKVC PQKECGWKEQ IEPPAPKE

References

[1]

"Characterization of ST-4821 complex, a unique Neisseria meningitidis clone." Peng J., Yang L., Yang F., Yang J., Yan Y., Nie H., Zhang X., Xiong Z., Jiang Y., Cheng F., Xu X., Chen S., Sun L., Li W., Shen Y., Shao Z., Liang X., Xu J., Jin Q. Genomics 91:78-87(2008) [PubMed: 18031983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000381 Genomic DNA. Translation: ABX74149.1.
RefSeq	YP_001600116.1. NC_010120.1.
3D structure databases
ProteinModelPortal	A9M455.
ModBase	Search...
Protein-protein interaction databases
STRING	A9M455.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000012632; EBNEIP00000012174; EBNEIG00000012632.
GeneID	5797011.
GenomeReviews	Gene locus NMCC_2030 in contig CP000381_GR.
KEGG	nmn:NMCC_2030.
PATRIC	20349241. VBINeiMen117761_2506.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000020417.
HOGENOM	HBG512935.
OMA	QELDETC.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A9M455_NEIM0

Accession

Primary (citable) accession number: A9M455

Entry history

Integrated into UniProtKB/TrEMBL:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)