A9M375 (SYT_NEIM0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	NMCC_0680

Organism

Neisseria meningitidis serogroup C (strain 053442) [Complete proteome] [HAMAP]

Taxonomic identifier

374833 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm By similarity HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000077365
Regions
	Region	242 – 533	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	384	1	Zinc; catalytic By similarity
	Metal binding	510	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9M375 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 1A4917735AA1D9A3	FASTA	637	72,580
	10 20 30 40 50 60 MLNITLPDGS VRQYESPVTV AQIAASIGAG LAKAAVAGKV NGKLVDACDP ITEDSSVQII 70 80 90 100 110 120 TPKDQEGIEI IRHSCAHLVG HAVKQLYPNA KMVIGPVIEE GFYYDIATEK PFTPEDVAAI 130 140 150 160 170 180 EARMKELIAQ DYDVVKIMTP RAEAIKIFQE RGEEYKLRLI DDMPEVEAMG MYHHQEYVDM 190 200 210 220 230 240 CRGPHVPNTR FLKNFKLTKL AGAYWRGDSN NEMLQRIYGT AWATKDELKA YIQRIEEAEK 250 260 270 280 290 300 RDHRKLGKQL DLFHLQDEAP GMVFWHPKGW ALWQVIEQHM RKELNAAGYK EVKTPQIMDK 310 320 330 340 350 360 TFWEKSGHWD NYKDNMFVTS SEKREYAVKP MNCPGHVQIF NNGLRSYRDL PMRLAEFGSC 370 380 390 400 410 420 HRNEPSGALH GLMRVRGFVQ DDAHIFCTED QIVSEARAFN ELLVRIYKQF GFHDVSVKLS 430 440 450 460 470 480 LRPEKRAGSD DVWDKAEQGL REALTACGVE WGELPGEGAF YGPKIEYHVR DALGRSWQCG 490 500 510 520 530 540 TLQLDFVLPE RLGAEYVTEN NDRARPVMLH RAILGSLERF IGILIENHAG SFPLWLAPVQ 550 560 570 580 590 600 LVIMNITENQ ADYCREVAAK LQAAGFRAEL DLRNEKIGYK IRDNSQYRFP YQIVVGDKEK 610 620 630 QENKVAVRRK AEDLGSLDLD DFIAQLQQEI TDALVNH

References

[1]

"Characterization of ST-4821 complex, a unique Neisseria meningitidis clone." Peng J., Yang L., Yang F., Yang J., Yan Y., Nie H., Zhang X., Xiong Z., Jiang Y., Cheng F., Xu X., Chen S., Sun L., Li W., Shen Y., Shao Z., Liang X., Xu J., Jin Q. Genomics 91:78-87(2008) [PubMed: 18031983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 053442.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000381 Genomic DNA. Translation: ABX72875.1.
RefSeq	YP_001598829.1. NC_010120.1.
3D structure databases
ProteinModelPortal	A9M375.
SMR	A9M375. Positions 4-630.
ModBase	Search...
Protein-protein interaction databases
STRING	A9M375.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000011664; EBNEIP00000011206; EBNEIG00000011664.
GeneID	5796620.
GenomeReviews	Gene locus NMCC_0680 in contig CP000381_GR.
KEGG	nmn:NMCC_0680.
PATRIC	20345803. VBINeiMen117761_0826.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000021120.
HOGENOM	HBG352811.
OMA	VGSDALW.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	NMEN374833:NMCC_0680-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_NEIM0

Accession

Primary (citable) accession number: A9M375

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program