A9L0E9 (GLO2_SHEB9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Sbal195_2059

Organism

Shewanella baltica (strain OS195) [Complete proteome] [HAMAP]

Taxonomic identifier

399599 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000144805
Sites
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	57	1	Zinc 1 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	60	1	Zinc 2 By similarity
	Metal binding	117	1	Zinc 1 By similarity
	Metal binding	134	1	Zinc 1 By similarity
	Metal binding	134	1	Zinc 2 By similarity
	Metal binding	172	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9L0E9 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: CF3D9F15C6BBD0AC	FASTA	263	29,145
	10 20 30 40 50 60 MLTITAIKAF NDNYIWVLQQ QPHTQVYVVD PGDASVVIDY LEANQLTLVG ILLTHHHNDH 70 80 90 100 110 120 TGGVAELQAY SQDRLTVYGP DNEKIEGITH PLHATAQPRF TLDYISGELQ VLDVPGHTAG 130 140 150 160 170 180 HIAYVIADAL FCGDTLFSGG CGRLFEGTPA QMLNSLQQLA QLPAYTRVYC AHEYTLSNLK 190 200 210 220 230 240 FALAVNPNNC ALQDYNERAI ALRRQDKATI PSTIALERAI NPFLRASDTE IVGSIKQHFS 250 260 DLSHANLDEL GGFTLLRQWK DNF

References

[1]

"Complete sequence of chromosome of Shewanella baltica OS195." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. , Brettar I., Rodrigues J., Konstantinidis K., Klappenbach J., Hofle M., Tiedje J., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OS195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000891 Genomic DNA. Translation: ABX49229.1.
RefSeq	YP_001554489.1. NC_009997.1.
3D structure databases
ProteinModelPortal	A9L0E9.
ModBase	Search...
Protein-protein interaction databases
STRING	A9L0E9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5753803.
GenomeReviews	Gene locus Sbal195_2059 in contig CP000891_GR.
KEGG	sbn:Sbal195_2059.
PATRIC	23470251. VBISheBal33754_2131.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	NILWALN.
ProtClustDB	CLSK906771.
Enzyme and pathway databases
BioCyc	SBAL399599:SBAL195_2059-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_SHEB9

Accession

Primary (citable) accession number: A9L0E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program