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A9L001 (SYL_SHEB9) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Sbal195_3447
OrganismShewanella baltica (strain OS195) [Complete proteome] [HAMAP]
Taxonomic identifier399599 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length859 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABX50609.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 859859Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334815

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9L001 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 28FDE3FEAEF8BC5F

FASTA85996,596
        10         20         30         40         50         60 
MQEQYNPSEI EALVQKHWHD NKTFEVTEDA NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVARFQRLQG KNVLQPIGWD SFGLPAENAA INNKTAPAPW TYENIEYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WSREIATCTP EYYRWEQWFF TKLYEKGLVY KKTASVNWCP NDETVLANEQ VQDGCCWRCD 

       190        200        210        220        230        240 
TPVEQKEIPQ WFIKITAYAE ELLNDIDTLD GWPDQVKTMQ RNWIGRSEGV EMTFGVAGHD 

       250        260        270        280        290        300 
KSFDIYTTRP DTLMGVTYVA IAAGHPLAEI AAQTNPELAA FIDECKNSTT SEAELATMEK 

       310        320        330        340        350        360 
RGVATGLFAI HPITGKQVPI WAANFVLMNY GTGAVMSVPG HDQRDFEFAK KYGLAIEAVI 

       370        380        390        400        410        420 
KPVDGDVDIS EAAYTEKGVL FNSGEFDGLD FEAGFNAIAN KLVAEGKGKR QVNYRLRDWG 

       430        440        450        460        470        480 
VSRQRYWGAP IPMVTLADGT VIPTPADQLP VLLPEDVVMD GIQSPIKADK EWAKTQVNGQ 

       490        500        510        520        530        540 
DALRETDTFD TFMESSWYYA RYCSPHADEM LDPAKANYWL PVDQYIGGIE HACMHLLYFR 

       550        560        570        580        590        600 
FFHKLLRDAG LVNSNEPAKQ LLTQGMVLAD AFYYINEKGA RVWVSPLDVA TTEKDDKGRI 

       610        620        630        640        650        660 
TKAIDKDGNE LVYTGMSKMS KSKNNGIDPQ VMVEKYGADT VRLFMMFASP PELTLEWQES 

       670        680        690        700        710        720 
GVEGAHRFIK RLWKLANEHV NQDNSEALDA SKLTSDQKAL RREVHKTIAK VTDDIGRRQM 

       730        740        750        760        770        780 
FNTAVAAVME LMNHLQKAPQ TTGQDNAIIG EALSAIVRLL YPIIPHVSFN LWNELGNASN 

       790        800        810        820        830        840 
IEDSQWPVVD EAALVEDSKL IVVQVNGKVR AKITVAADAD KESVEALGMS DEHVIKYLDG 

       850 
LTVRKVIYVP GKLLSIVAN 

« Hide

References

[1]"Complete sequence of chromosome of Shewanella baltica OS195."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. expand/collapse author list , Brettar I., Rodrigues J., Konstantinidis K., Klappenbach J., Hofle M., Tiedje J., Richardson P.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS195.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000891 Genomic DNA. Translation: ABX50609.1. Different initiation.
RefSeqYP_001555869.1. NC_009997.1.

3D structure databases

ProteinModelPortalA9L001.
SMRA9L001. Positions 228-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399599.Sbal195_3447.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX50609; ABX50609; Sbal195_3447.
GeneID5755260.
KEGGsbn:Sbal195_3447.
PATRIC23473281. VBISheBal33754_3576.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSBAL399599:GH6B-3569-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SHEB9
AccessionPrimary (citable) accession number: A9L001
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries