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A9KY60

- BIOB_SHEB9

UniProt

A9KY60 - BIOB_SHEB9

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Protein
Biotin synthase
Gene
bioB, Sbal195_1795
Organism
Shewanella baltica (strain OS195)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi100 – 1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi191 – 1911Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi263 – 2631Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSBAL399599:GH6B-1846-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Sbal195_1795
OrganismiShewanella baltica (strain OS195)
Taxonomic identifieri399599 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000000770: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Biotin synthaseUniRule annotation
PRO_0000381616Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi399599.Sbal195_1795.

Structurei

3D structure databases

ProteinModelPortaliA9KY60.
SMRiA9KY60. Positions 7-317.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A9KY60-1 [UniParc]FASTAAdd to Basket

« Hide

MSQLQVRHDW KREEIEALFA LPMNDLLFKA HSIHREVYDP NEVQISRLLS    50
IKTGACPEDC KYCPQSARYD TGLEKERLLA METVLTEARS AKAAGASRFC 100
MGAAWRNPKE KDMPYLKQMV QEVKALGMET CMTLGMLSED QANDLASAGL 150
DYYNHNLDTS PEYYGDVITT RTYQNRLDTL TNVRASGMKV CSGGIVGMGE 200
KATDRAGLLQ QLANLPQHPD SVPINMLVKV AGTPFEKLDD LDPLEFVRTI 250
AVARILMPLS RVRLSAGREN MSDELQAMCF FAGANSIFYG CKLLTTPNPE 300
ESDDMGLFRR LGLRPEQGAA AKLEEESAVL AKAAAYQDKS SAQFYDAGAL 350
Length:350
Mass (Da):38,783
Last modified:February 5, 2008 - v1
Checksum:i9B6A317D5553E1AC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000891 Genomic DNA. Translation: ABX48966.1.
RefSeqiYP_001554226.1. NC_009997.1.

Genome annotation databases

EnsemblBacteriaiABX48966; ABX48966; Sbal195_1795.
GeneIDi5753538.
KEGGisbn:Sbal195_1795.
PATRICi23469694. VBISheBal33754_1854.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000891 Genomic DNA. Translation: ABX48966.1 .
RefSeqi YP_001554226.1. NC_009997.1.

3D structure databases

ProteinModelPortali A9KY60.
SMRi A9KY60. Positions 7-317.
ModBasei Search...

Protein-protein interaction databases

STRINGi 399599.Sbal195_1795.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX48966 ; ABX48966 ; Sbal195_1795 .
GeneIDi 5753538.
KEGGi sbn:Sbal195_1795.
PATRICi 23469694. VBISheBal33754_1854.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci SBAL399599:GH6B-1846-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OS195.

Entry informationi

Entry nameiBIOB_SHEB9
AccessioniPrimary (citable) accession number: A9KY60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 5, 2008
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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