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Protein

Nigerose phosphorylase

Gene

Cphy_1874

Organism
Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Lachnoclostridium phytofermentans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorolysis of nigerose. Also shows a weak activity on kojibiose.1 Publication

Catalytic activityi

3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate = D-glucose + beta-D-glucose 1-phosphate.1 Publication

Enzyme regulationi

Does not require divalent metal ions.1 Publication

Kineticsi

kcat is 67 sec(-1) for nigerose. kcat is 110 sec(-1) for D-glucose.

  1. KM=1.7 mM for nigerose1 Publication
  2. KM=0.2 mM for phosphate1 Publication
  3. KM=3.3 mM for D-glucose1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 40 degrees Celsius.1 Publication

    GO - Molecular functioni

    • carbohydrate binding Source: InterPro
    • phosphorylase activity Source: CACAO

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.279. 10424.

    Protein family/group databases

    CAZyiGH65. Glycoside Hydrolase Family 65.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nigerose phosphorylase (EC:2.4.1.2791 Publication)
    Gene namesi
    Ordered Locus Names:Cphy_1874
    OrganismiClostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Lachnoclostridium phytofermentans)
    Taxonomic identifieri357809 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceae
    Proteomesi
    • UP000000370 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004187231 – 753Nigerose phosphorylaseAdd BLAST753

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi357809.Cphy_1874.

    Structurei

    3D structure databases

    ProteinModelPortaliA9KT32.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 65 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105C71. Bacteria.
    COG1554. LUCA.
    HOGENOMiHOG000055022.
    KOiK18783.
    OMAiFNDWQIH.
    OrthoDBiPOG091H075X.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    2.70.98.40. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR005194. Glyco_hydro_65_C.
    IPR005195. Glyco_hydro_65_M.
    IPR005196. Glyco_hydro_65_N.
    IPR017045. Malt_Pase/Glycosyl_Hdrlase.
    [Graphical view]
    PfamiPF03633. Glyco_hydro_65C. 1 hit.
    PF03632. Glyco_hydro_65m. 1 hit.
    PF03636. Glyco_hydro_65N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036289. Glycosyl_hydrolase_malt_phosph. 1 hit.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF74650. SSF74650. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A9KT32-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNWTLTNSSL DKDSITSNGN RFLIGNGYLG IRGTLEEYRK EYFPAINLAG
    60 70 80 90 100
    IYDQVGEGWR EPLNAPNALY TRIEVDEVEY QLPKIEPRYH ELSLDYRHGI
    110 120 130 140 150
    LDRQTVWASN KGTIIVKSSR FASMKEKHLV VLNYSITADY DCEIIVYTGI
    160 170 180 190 200
    DGSVWDIHGP HYDKVEFQKQ LLRHNETEIL NEKYLLESES KWNGHRLSIA
    210 220 230 240 250
    AQTHENKDIV YVTEDIICNK EAKIELIESD KECLRKLTFH GKAKEEINFT
    260 270 280 290 300
    KYITVFTSKD CVDYKEQSIK IVNHAKDTGY ERLQEEHKNV WEQLWNISEV
    310 320 330 340 350
    TIEGDDEAND ALNYSLYHLH CIAPRHSKSL SIAARGLSGQ TYKGAVFWDT
    360 370 380 390 400
    EMFMLDFFLY TQPEVAKTLL RYRIDTLEGA KKKAKLYGYE GAFYAWESQE
    410 420 430 440 450
    GGYDACSDYN VTDVFTKRPM RTYFKDKQVH ISSAIVYGIR SYLNYTNDFS
    460 470 480 490 500
    ILAEGGAETI LECAKFYYSL LEKKIGKEYY EIHDVIGPDE YHERVNNNAY
    510 520 530 540 550
    TNRMAKLTFE TAIDILDHEK NKDEEFYIKL LKQYEIKDLL DKLKDACNKL
    560 570 580 590 600
    YIPKPKDNSD LIEQFDGFFE LEDVSLEEVR SRLLHEKEYW GGAYGVASHT
    610 620 630 640 650
    QVIKQADVVT MLVLFKEEYQ REVLQQNLNY YEPRTEHGSS LSACMYSLLY
    660 670 680 690 700
    CMCDQPQYAY PFFMKSALAD WNGKGKEWAG LVYIGGTHPA AAGGAYMTAI
    710 720 730 740 750
    KGFGGFQIEN GVIKATPRLP KHWVRLKYRV LYQGAIYEID ASKEQVSISK

    IEM
    Length:753
    Mass (Da):87,173
    Last modified:February 5, 2008 - v1
    Checksum:iD8AF1D004AF8FCC1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000885 Genomic DNA. Translation: ABX42243.1.
    RefSeqiWP_012199897.1. NC_010001.1.

    Genome annotation databases

    EnsemblBacteriaiABX42243; ABX42243; Cphy_1874.
    KEGGicpy:Cphy_1874.
    PATRICi19503406. VBICloPhy16213_1942.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000885 Genomic DNA. Translation: ABX42243.1.
    RefSeqiWP_012199897.1. NC_010001.1.

    3D structure databases

    ProteinModelPortaliA9KT32.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi357809.Cphy_1874.

    Protein family/group databases

    CAZyiGH65. Glycoside Hydrolase Family 65.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABX42243; ABX42243; Cphy_1874.
    KEGGicpy:Cphy_1874.
    PATRICi19503406. VBICloPhy16213_1942.

    Phylogenomic databases

    eggNOGiENOG4105C71. Bacteria.
    COG1554. LUCA.
    HOGENOMiHOG000055022.
    KOiK18783.
    OMAiFNDWQIH.
    OrthoDBiPOG091H075X.

    Enzyme and pathway databases

    BRENDAi2.4.1.279. 10424.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    2.70.98.40. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR005194. Glyco_hydro_65_C.
    IPR005195. Glyco_hydro_65_M.
    IPR005196. Glyco_hydro_65_N.
    IPR017045. Malt_Pase/Glycosyl_Hdrlase.
    [Graphical view]
    PfamiPF03633. Glyco_hydro_65C. 1 hit.
    PF03632. Glyco_hydro_65m. 1 hit.
    PF03636. Glyco_hydro_65N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036289. Glycosyl_hydrolase_malt_phosph. 1 hit.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF74650. SSF74650. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNIGP_CLOPH
    AccessioniPrimary (citable) accession number: A9KT32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: February 5, 2008
    Last modified: November 2, 2016
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.