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Protein

Nigerose phosphorylase

Gene

Cphy_1874

Organism
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorolysis of nigerose. Also shows a weak activity on kojibiose.1 Publication

Catalytic activityi

3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate = D-glucose + beta-D-glucose 1-phosphate.1 Publication

Enzyme regulationi

Does not require divalent metal ions.1 Publication

Kineticsi

kcat is 67 sec(-1) for nigerose. kcat is 110 sec(-1) for D-glucose.
  1. KM=1.7 mM for nigerose1 Publication
  2. KM=0.2 mM for phosphate1 Publication
  3. KM=3.3 mM for D-glucose1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 40 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei490Proton donorBy similarity1

    GO - Molecular functioni

    • carbohydrate binding Source: InterPro
    • phosphorylase activity Source: CACAO

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciCPHY357809:G1GAJ-1936-MONOMER
    BRENDAi2.4.1.279 10424

    Protein family/group databases

    CAZyiGH65 Glycoside Hydrolase Family 65

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nigerose phosphorylase (EC:2.4.1.2791 Publication)
    Gene namesi
    Ordered Locus Names:Cphy_1874
    OrganismiLachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
    Taxonomic identifieri357809 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceae
    Proteomesi
    • UP000000370 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm 1 Publication

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004187231 – 753Nigerose phosphorylaseAdd BLAST753

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi357809.Cphy_1874

    Structurei

    3D structure databases

    ProteinModelPortaliA9KT32
    SMRiA9KT32
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni348 – 349Substrate bindingBy similarity2
    Regioni604 – 605Substrate bindingBy similarity2

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 65 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105C71 Bacteria
    COG1554 LUCA
    HOGENOMiHOG000055022
    KOiK18783
    OMAiECAKFYY
    OrthoDBiPOG091H075X

    Family and domain databases

    Gene3Di1.50.10.10, 1 hit
    2.70.98.40, 1 hit
    InterProiView protein in InterPro
    IPR008928 6-hairpin_glycosidase_sf
    IPR012341 6hp_glycosidase-like_sf
    IPR011013 Gal_mutarotase_sf_dom
    IPR005194 Glyco_hydro_65_C
    IPR005195 Glyco_hydro_65_M
    IPR005196 Glyco_hydro_65_N
    IPR037018 Glyco_hydro_65_N_sf
    IPR017045 Malt_Pase/Glycosyl_Hdrlase
    PfamiView protein in Pfam
    PF03633 Glyco_hydro_65C, 1 hit
    PF03632 Glyco_hydro_65m, 1 hit
    PF03636 Glyco_hydro_65N, 1 hit
    PIRSFiPIRSF036289 Glycosyl_hydrolase_malt_phosph, 1 hit
    SUPFAMiSSF48208 SSF48208, 1 hit
    SSF74650 SSF74650, 1 hit

    Sequencei

    Sequence statusi: Complete.

    A9KT32-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNWTLTNSSL DKDSITSNGN RFLIGNGYLG IRGTLEEYRK EYFPAINLAG
    60 70 80 90 100
    IYDQVGEGWR EPLNAPNALY TRIEVDEVEY QLPKIEPRYH ELSLDYRHGI
    110 120 130 140 150
    LDRQTVWASN KGTIIVKSSR FASMKEKHLV VLNYSITADY DCEIIVYTGI
    160 170 180 190 200
    DGSVWDIHGP HYDKVEFQKQ LLRHNETEIL NEKYLLESES KWNGHRLSIA
    210 220 230 240 250
    AQTHENKDIV YVTEDIICNK EAKIELIESD KECLRKLTFH GKAKEEINFT
    260 270 280 290 300
    KYITVFTSKD CVDYKEQSIK IVNHAKDTGY ERLQEEHKNV WEQLWNISEV
    310 320 330 340 350
    TIEGDDEAND ALNYSLYHLH CIAPRHSKSL SIAARGLSGQ TYKGAVFWDT
    360 370 380 390 400
    EMFMLDFFLY TQPEVAKTLL RYRIDTLEGA KKKAKLYGYE GAFYAWESQE
    410 420 430 440 450
    GGYDACSDYN VTDVFTKRPM RTYFKDKQVH ISSAIVYGIR SYLNYTNDFS
    460 470 480 490 500
    ILAEGGAETI LECAKFYYSL LEKKIGKEYY EIHDVIGPDE YHERVNNNAY
    510 520 530 540 550
    TNRMAKLTFE TAIDILDHEK NKDEEFYIKL LKQYEIKDLL DKLKDACNKL
    560 570 580 590 600
    YIPKPKDNSD LIEQFDGFFE LEDVSLEEVR SRLLHEKEYW GGAYGVASHT
    610 620 630 640 650
    QVIKQADVVT MLVLFKEEYQ REVLQQNLNY YEPRTEHGSS LSACMYSLLY
    660 670 680 690 700
    CMCDQPQYAY PFFMKSALAD WNGKGKEWAG LVYIGGTHPA AAGGAYMTAI
    710 720 730 740 750
    KGFGGFQIEN GVIKATPRLP KHWVRLKYRV LYQGAIYEID ASKEQVSISK

    IEM
    Length:753
    Mass (Da):87,173
    Last modified:February 5, 2008 - v1
    Checksum:iD8AF1D004AF8FCC1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000885 Genomic DNA Translation: ABX42243.1
    RefSeqiWP_012199897.1, NC_010001.1

    Genome annotation databases

    EnsemblBacteriaiABX42243; ABX42243; Cphy_1874
    KEGGicpy:Cphy_1874

    Similar proteinsi

    Entry informationi

    Entry nameiNIGP_LACP7
    AccessioniPrimary (citable) accession number: A9KT32
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: February 5, 2008
    Last modified: March 28, 2018
    This is version 58 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health