A9KRZ3 (EFG_CLOPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	Cphy_0237

Organism

Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) [Complete proteome] [HAMAP]

Taxonomic identifier

357809 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	Elongation factor G HAMAP MF_00054_B		PRO_0000335844
Regions
	Nucleotide binding	18 – 25	8	GTP By similarity
	Nucleotide binding	94 – 98	5	GTP By similarity
	Nucleotide binding	148 – 151	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9KRZ3 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 60FC86988827A3AA	FASTA	705	78,189
	10 20 30 40 50 60 MAGREYPLER TRNIGIMAHI DAGKTTLSER ILYYTGVNYK IGDTHEGTAT MDWMEQEQER 70 80 90 100 110 120 GITITSAATT CHWTLELEHK KAPGALEHRI NLIDTPGHVD FTVEVERSLR VLDSAVGVFC 130 140 150 160 170 180 AKGGVEPQSE TVWRQADKYN VPRMAFVNKM DISGANFFNV VDMIKSRLGK NAVPIQLPIG 190 200 210 220 230 240 KEDTFKGVID LFEMKAYYYL DDKGEQIEIK EIPDDMKDQA EEYRAAMIES ICETDDDLIE 250 260 270 280 290 300 AFLEGNEPSN EELKKALRNA TISVQIIPVL CGSAYRNKGV QKLLDAVIEF MPAPTDIEDI 310 320 330 340 350 360 KGFDEEGNEI HRISSDEEPF AALAFKIMAD PFVGKLAFFR VYSGTLNAGS YVLNATKNKK 370 380 390 400 410 420 ERVGRILQMH ANKREDLDKV YSGDIAAAVG FKFTSTGDTI CDEKHPVVLE AMEFPEPVID 430 440 450 460 470 480 VAIEPKTKAG QDKMGEALAK LAEEDPTFRV RTNEETGQTI IAGMGELHLE IIVDRLLREF 490 500 510 520 530 540 KVEANVGAPQ VAYKEGFTKE VDIDSKYAKQ SGGRGQYGHC KVKFSPMDVN GEKVFEFVST 550 560 570 580 590 600 VVGGAIPKEY IPAVQAGIED AMKCGVLGGY PVLGVRANCY DGSYHEVDSN EMAFKIAGSM 610 620 630 640 650 660 AFKDAMHKAG PILLEPIMRV EVTVPDDYMG DVIGDISSRR GRIEGTEDNN GSKIIRGFVP 670 680 690 700 LSEMFGYSTT LRSKTQGRGA YSMFFSTYEP VPKNVQEKVL SNKTK

References

[1]

"Complete genome sequence of Clostridium phytofermentans ISDg." Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L., LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C. , Han C., Kuske C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.A., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700394 / DSM 18823 / ISDg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000885 Genomic DNA. Translation: ABX40624.1.
RefSeq	YP_001557363.1. NC_010001.1.
3D structure databases
ProteinModelPortal	A9KRZ3.
SMR	A9KRZ3. Positions 5-700.
ModBase	Search...
Protein-protein interaction databases
STRING	A9KRZ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5745105.
GenomeReviews	Gene locus Cphy_0237 in contig CP000885_GR.
KEGG	cpy:Cphy_0237.
PATRIC	19499962. VBICloPhy16213_0244.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK00007.
Enzyme and pathway databases
BioCyc	CPHY357809:CPHY_0237-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_CLOPH

Accession

Primary (citable) accession number: A9KRZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program