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A9KRX7

- SYE_CLOPH

UniProt

A9KRX7 - SYE_CLOPH

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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Lachnoclostridium phytofermentans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091ZincUniRule annotation
Metal bindingi111 – 1111ZincUniRule annotation
Metal bindingi140 – 1401ZincUniRule annotation
Metal bindingi142 – 1421ZincUniRule annotation
Binding sitei260 – 2601ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPHY357809:GHCL-242-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Cphy_0221
OrganismiClostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Lachnoclostridium phytofermentans)
Taxonomic identifieri357809 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceae
ProteomesiUP000000370: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Glutamate--tRNA ligasePRO_0000330965Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi357809.Cphy_0221.

Structurei

3D structure databases

ProteinModelPortaliA9KRX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionAdd
BLAST
Motifi257 – 2615"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiWLPEEMG.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9KRX7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKVRTRFAP SPTGRMHVGN LRTALYEYLI AKHEGGDFIL RIEDTDQERL
60 70 80 90 100
VEGATEIIYR TIAKTGLIHD EGPDKDKGFG PYVQSERQAT GIYLKYAKEL
110 120 130 140 150
VENGEAYYCF CTKERLETLK SAVGEEDGES KEITKYDKHC LHLSKEEVEA
160 170 180 190 200
NLAAGMPYVI RQNMPTEGTT SFTDAIYGTI TVENSELDDM ILIKSDGFPT
210 220 230 240 250
YNFANVIDDH FMEITHVVRG NEYLSSTPKY TRLYKAFGWE EPVYIHCPLI
260 270 280 290 300
TNEEHQKLSK RSGHSSFEDL IEQGFVTEAI VNFIALLGWS STTNEEIFSL
310 320 330 340 350
EELVREFDYT HINKSPSVFD MNKLRWMNGE YIKRMDSEKY YEYALPQIKK
360 370 380 390 400
VVTKDYDLKF IADLVKTRIE TFLDIAEMID FFDELPEYDI AMYTHKKMKT
410 420 430 440 450
DSENSLKVLQ DVLPRFEELT CYSVSSIESV LMGYIAENGI KNGQGLWPVR
460 470 480 490
TAVSGKQSTP GGAYEIMSIL GKEESLRRIR IAIDKLSSSL
Length:490
Mass (Da):56,076
Last modified:February 5, 2008 - v1
Checksum:iB40D2E85011A7CD6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000885 Genomic DNA. Translation: ABX40608.1.
RefSeqiWP_012198251.1. NC_010001.1.
YP_001557347.1. NC_010001.1.

Genome annotation databases

EnsemblBacteriaiABX40608; ABX40608; Cphy_0221.
GeneIDi5745089.
KEGGicpy:Cphy_0221.
PATRICi19499930. VBICloPhy16213_0228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000885 Genomic DNA. Translation: ABX40608.1 .
RefSeqi WP_012198251.1. NC_010001.1.
YP_001557347.1. NC_010001.1.

3D structure databases

ProteinModelPortali A9KRX7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 357809.Cphy_0221.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX40608 ; ABX40608 ; Cphy_0221 .
GeneIDi 5745089.
KEGGi cpy:Cphy_0221.
PATRICi 19499930. VBICloPhy16213_0228.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K01885.
OMAi WLPEEMG.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci CPHY357809:GHCL-242-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700394 / DSM 18823 / ISDg.

Entry informationi

Entry nameiSYE_CLOPH
AccessioniPrimary (citable) accession number: A9KRX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 5, 2008
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3