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A9KRX7

- SYE_CLOPH

UniProt

A9KRX7 - SYE_CLOPH

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Protein
Glutamate--tRNA ligase
Gene
gltX, Cphy_0221
Organism
Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091Zinc By similarity
Metal bindingi111 – 1111Zinc By similarity
Metal bindingi140 – 1401Zinc By similarity
Metal bindingi142 – 1421Zinc By similarity
Binding sitei260 – 2601ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPHY357809:GHCL-242-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:Cphy_0221
OrganismiClostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
Taxonomic identifieri357809 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceae
ProteomesiUP000000370: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Glutamate--tRNA ligaseUniRule annotation
PRO_0000330965Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi357809.Cphy_0221.

Structurei

3D structure databases

ProteinModelPortaliA9KRX7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionUniRule annotation
Add
BLAST
Motifi257 – 2615"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiWLPEEMG.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9KRX7-1 [UniParc]FASTAAdd to Basket

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MAKVRTRFAP SPTGRMHVGN LRTALYEYLI AKHEGGDFIL RIEDTDQERL    50
VEGATEIIYR TIAKTGLIHD EGPDKDKGFG PYVQSERQAT GIYLKYAKEL 100
VENGEAYYCF CTKERLETLK SAVGEEDGES KEITKYDKHC LHLSKEEVEA 150
NLAAGMPYVI RQNMPTEGTT SFTDAIYGTI TVENSELDDM ILIKSDGFPT 200
YNFANVIDDH FMEITHVVRG NEYLSSTPKY TRLYKAFGWE EPVYIHCPLI 250
TNEEHQKLSK RSGHSSFEDL IEQGFVTEAI VNFIALLGWS STTNEEIFSL 300
EELVREFDYT HINKSPSVFD MNKLRWMNGE YIKRMDSEKY YEYALPQIKK 350
VVTKDYDLKF IADLVKTRIE TFLDIAEMID FFDELPEYDI AMYTHKKMKT 400
DSENSLKVLQ DVLPRFEELT CYSVSSIESV LMGYIAENGI KNGQGLWPVR 450
TAVSGKQSTP GGAYEIMSIL GKEESLRRIR IAIDKLSSSL 490
Length:490
Mass (Da):56,076
Last modified:February 5, 2008 - v1
Checksum:iB40D2E85011A7CD6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000885 Genomic DNA. Translation: ABX40608.1.
RefSeqiYP_001557347.1. NC_010001.1.

Genome annotation databases

EnsemblBacteriaiABX40608; ABX40608; Cphy_0221.
GeneIDi5745089.
KEGGicpy:Cphy_0221.
PATRICi19499930. VBICloPhy16213_0228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000885 Genomic DNA. Translation: ABX40608.1 .
RefSeqi YP_001557347.1. NC_010001.1.

3D structure databases

ProteinModelPortali A9KRX7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 357809.Cphy_0221.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX40608 ; ABX40608 ; Cphy_0221 .
GeneIDi 5745089.
KEGGi cpy:Cphy_0221.
PATRICi 19499930. VBICloPhy16213_0228.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K01885.
OMAi WLPEEMG.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci CPHY357809:GHCL-242-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700394 / DSM 18823 / ISDg.

Entry informationi

Entry nameiSYE_CLOPH
AccessioniPrimary (citable) accession number: A9KRX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 5, 2008
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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