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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109ZincUniRule annotation1
Metal bindingi111ZincUniRule annotation1
Metal bindingi140ZincUniRule annotation1
Metal bindingi142ZincUniRule annotation1
Binding sitei260ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPHY357809:G1GAJ-241-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Cphy_0221
OrganismiLachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
Taxonomic identifieri357809 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceae
Proteomesi
  • UP000000370 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003309651 – 490Glutamate--tRNA ligaseAdd BLAST490

Proteomic databases

PRIDEiA9KRX7

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi357809.Cphy_0221

Structurei

3D structure databases

ProteinModelPortaliA9KRX7
SMRiA9KRX7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionUniRule annotationAdd BLAST11
Motifi257 – 261"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A9KRX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVRTRFAP SPTGRMHVGN LRTALYEYLI AKHEGGDFIL RIEDTDQERL
60 70 80 90 100
VEGATEIIYR TIAKTGLIHD EGPDKDKGFG PYVQSERQAT GIYLKYAKEL
110 120 130 140 150
VENGEAYYCF CTKERLETLK SAVGEEDGES KEITKYDKHC LHLSKEEVEA
160 170 180 190 200
NLAAGMPYVI RQNMPTEGTT SFTDAIYGTI TVENSELDDM ILIKSDGFPT
210 220 230 240 250
YNFANVIDDH FMEITHVVRG NEYLSSTPKY TRLYKAFGWE EPVYIHCPLI
260 270 280 290 300
TNEEHQKLSK RSGHSSFEDL IEQGFVTEAI VNFIALLGWS STTNEEIFSL
310 320 330 340 350
EELVREFDYT HINKSPSVFD MNKLRWMNGE YIKRMDSEKY YEYALPQIKK
360 370 380 390 400
VVTKDYDLKF IADLVKTRIE TFLDIAEMID FFDELPEYDI AMYTHKKMKT
410 420 430 440 450
DSENSLKVLQ DVLPRFEELT CYSVSSIESV LMGYIAENGI KNGQGLWPVR
460 470 480 490
TAVSGKQSTP GGAYEIMSIL GKEESLRRIR IAIDKLSSSL
Length:490
Mass (Da):56,076
Last modified:February 5, 2008 - v1
Checksum:iB40D2E85011A7CD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000885 Genomic DNA Translation: ABX40608.1
RefSeqiWP_012198251.1, NC_010001.1

Genome annotation databases

EnsemblBacteriaiABX40608; ABX40608; Cphy_0221
KEGGicpy:Cphy_0221

Similar proteinsi

Entry informationi

Entry nameiSYE_LACP7
AccessioniPrimary (citable) accession number: A9KRX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 5, 2008
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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