A9KQC2 (GLPK_CLOPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Cphy_0041

Organism

Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) [Complete proteome] [HAMAP]

Taxonomic identifier

357809 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium phytofermentans

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity. HAMAP-Rule MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP-Rule MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP-Rule MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycerol catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Glycerol kinase HAMAP-Rule MF_00186		PRO_1000077414
Regions
	Nucleotide binding	409 – 413	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	308	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9KQC2 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: AF7ABE5407A0B1E2	FASTA	499	55,312
	10 20 30 40 50 60 MEKYIMALDQ GTTSSRCILF NQRGEVCSVA QKEFTQVYPR TGWVEHRPMD IWSSQISVAA 70 80 90 100 110 120 EAMAAIGAKA EDIDSIGITN QRETTIVWDK NTGEPVYNAI VWQCHRTADM IEQLKKDGFD 130 140 150 160 170 180 SVIRKKTGLI PDAYFSATKI KWILDNVDGV RERAEAGSLL FGTVDTWIIW NLTKGRVHVT 190 200 210 220 230 240 DYTNASRTML FDIHNLCWDD EILTYFKIPK SMLPKVQASS SIFGHTEEGL LGGEILISGA 250 260 270 280 290 300 AGDQQAALFG QCCFEPGEVK NTYGTGCFLL MNTGDTAIES QNGLLTTIAA GDAGHIEYAL 310 320 330 340 350 360 EGSVFVAGAA IQWLRDEQRM IKKASQSEEY AKEVEDTNGV YVVPAFTGLG APYWNPYARG 370 380 390 400 410 420 TIVGITRGFS KEHMIRATLE SLAYQTVNVL HAMEQDSNIS LKSLRVDGGA SANNFLMQFQ 430 440 450 460 470 480 ADVLNTLVYR PQCIETTALG AAYLAGLATG YFKDRNEIKD NWTLGGTFSP QMDEQKRKEL 490 LKGWKRAVRC ALAWAEDIE

References

[1]

"Complete genome sequence of Clostridium phytofermentans ISDg." Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L., LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C. , Han C., Kuske C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.A., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700394 / DSM 18823 / ISDg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000885 Genomic DNA. Translation: ABX40431.1.
RefSeq	YP_001557170.1. NC_010001.1.
3D structure databases
ProteinModelPortal	A9KQC2.
SMR	A9KQC2. Positions 2-496.
ModBase	Search...
Protein-protein interaction databases
STRING	357809.Cphy_0041.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX40431; ABX40431; Cphy_0041.
GeneID	5744961.
KEGG	cpy:Cphy_0041.
PATRIC	19499534. VBICloPhy16213_0040.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HOG000222134.
KO	K00864.
OMA	ALYGQLC.
ProtClustDB	CLSK2796248.
Enzyme and pathway databases
BioCyc	CPHY357809:GHCL-130-MONOMER.
UniPathway	UPA00618; UER00672.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin.
InterPro	IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196:SF9. PTHR10196:SF9. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_CLOPH

Accession

Primary (citable) accession number: A9KQC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program