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Protein

Glycerol kinase

Gene

glpK

Organism
Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Lachnoclostridium phytofermentans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121SubstrateUniRule annotation
Binding sitei16 – 161ATPUniRule annotation
Binding sitei134 – 1341SubstrateUniRule annotation
Binding sitei265 – 2651ATPUniRule annotation
Binding sitei308 – 3081ATP; via carbonyl oxygenUniRule annotation
Binding sitei312 – 3121ATP; via amide nitrogenUniRule annotation
Binding sitei327 – 3271ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 143ATPUniRule annotation
Nucleotide bindingi409 – 4135ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPHY357809:GHCL-52-MONOMER.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:Cphy_0041
OrganismiClostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Lachnoclostridium phytofermentans)
Taxonomic identifieri357809 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceae
ProteomesiUP000000370 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Glycerol kinasePRO_1000077414Add
BLAST

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium).UniRule annotation

Protein-protein interaction databases

STRINGi357809.Cphy_0041.

Structurei

3D structure databases

ProteinModelPortaliA9KQC2.
SMRiA9KQC2. Positions 2-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate bindingUniRule annotation
Regioni243 – 2442Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0554.
HOGENOMiHOG000222134.
KOiK00864.
OMAiMARWEHA.
OrthoDBiEOG6RZB46.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9KQC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKYIMALDQ GTTSSRCILF NQRGEVCSVA QKEFTQVYPR TGWVEHRPMD
60 70 80 90 100
IWSSQISVAA EAMAAIGAKA EDIDSIGITN QRETTIVWDK NTGEPVYNAI
110 120 130 140 150
VWQCHRTADM IEQLKKDGFD SVIRKKTGLI PDAYFSATKI KWILDNVDGV
160 170 180 190 200
RERAEAGSLL FGTVDTWIIW NLTKGRVHVT DYTNASRTML FDIHNLCWDD
210 220 230 240 250
EILTYFKIPK SMLPKVQASS SIFGHTEEGL LGGEILISGA AGDQQAALFG
260 270 280 290 300
QCCFEPGEVK NTYGTGCFLL MNTGDTAIES QNGLLTTIAA GDAGHIEYAL
310 320 330 340 350
EGSVFVAGAA IQWLRDEQRM IKKASQSEEY AKEVEDTNGV YVVPAFTGLG
360 370 380 390 400
APYWNPYARG TIVGITRGFS KEHMIRATLE SLAYQTVNVL HAMEQDSNIS
410 420 430 440 450
LKSLRVDGGA SANNFLMQFQ ADVLNTLVYR PQCIETTALG AAYLAGLATG
460 470 480 490
YFKDRNEIKD NWTLGGTFSP QMDEQKRKEL LKGWKRAVRC ALAWAEDIE
Length:499
Mass (Da):55,312
Last modified:February 5, 2008 - v1
Checksum:iAF7ABE5407A0B1E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000885 Genomic DNA. Translation: ABX40431.1.
RefSeqiWP_012198074.1. NC_010001.1.
YP_001557170.1. NC_010001.1.

Genome annotation databases

EnsemblBacteriaiABX40431; ABX40431; Cphy_0041.
KEGGicpy:Cphy_0041.
PATRICi19499534. VBICloPhy16213_0040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000885 Genomic DNA. Translation: ABX40431.1.
RefSeqiWP_012198074.1. NC_010001.1.
YP_001557170.1. NC_010001.1.

3D structure databases

ProteinModelPortaliA9KQC2.
SMRiA9KQC2. Positions 2-496.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi357809.Cphy_0041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX40431; ABX40431; Cphy_0041.
KEGGicpy:Cphy_0041.
PATRICi19499534. VBICloPhy16213_0040.

Phylogenomic databases

eggNOGiCOG0554.
HOGENOMiHOG000222134.
KOiK00864.
OMAiMARWEHA.
OrthoDBiEOG6RZB46.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
BioCyciCPHY357809:GHCL-52-MONOMER.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700394 / DSM 18823 / ISDg.

Entry informationi

Entry nameiGLPK_CLOPH
AccessioniPrimary (citable) accession number: A9KQC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: May 27, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.