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A9KQC2 (GLPK_CLOPH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycerol kinase

EC=2.7.1.30
Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK
Gene names
Name:glpK
Ordered Locus Names:Cphy_0041
OrganismClostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) [Complete proteome] [HAMAP]
Taxonomic identifier357809 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate By similarity. HAMAP-Rule MF_00186

Catalytic activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP-Rule MF_00186

Enzyme regulation

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP) By similarity. HAMAP-Rule MF_00186

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP-Rule MF_00186

Subunit structure

Homotetramer and homodimer (in equilibrium) By similarity.

Sequence similarities

Belongs to the FGGY kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Glycerol kinase HAMAP-Rule MF_00186
PRO_1000077414

Regions

Nucleotide binding12 – 143ATP By similarity
Nucleotide binding409 – 4135ATP By similarity
Region82 – 832Substrate binding By similarity
Region243 – 2442Substrate binding By similarity

Sites

Binding site121Substrate By similarity
Binding site161ATP By similarity
Binding site1341Substrate By similarity
Binding site2651ATP By similarity
Binding site3081ATP; via carbonyl oxygen By similarity
Binding site3121ATP; via amide nitrogen By similarity
Binding site3271ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9KQC2 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: AF7ABE5407A0B1E2

FASTA49955,312
        10         20         30         40         50         60 
MEKYIMALDQ GTTSSRCILF NQRGEVCSVA QKEFTQVYPR TGWVEHRPMD IWSSQISVAA 

        70         80         90        100        110        120 
EAMAAIGAKA EDIDSIGITN QRETTIVWDK NTGEPVYNAI VWQCHRTADM IEQLKKDGFD 

       130        140        150        160        170        180 
SVIRKKTGLI PDAYFSATKI KWILDNVDGV RERAEAGSLL FGTVDTWIIW NLTKGRVHVT 

       190        200        210        220        230        240 
DYTNASRTML FDIHNLCWDD EILTYFKIPK SMLPKVQASS SIFGHTEEGL LGGEILISGA 

       250        260        270        280        290        300 
AGDQQAALFG QCCFEPGEVK NTYGTGCFLL MNTGDTAIES QNGLLTTIAA GDAGHIEYAL 

       310        320        330        340        350        360 
EGSVFVAGAA IQWLRDEQRM IKKASQSEEY AKEVEDTNGV YVVPAFTGLG APYWNPYARG 

       370        380        390        400        410        420 
TIVGITRGFS KEHMIRATLE SLAYQTVNVL HAMEQDSNIS LKSLRVDGGA SANNFLMQFQ 

       430        440        450        460        470        480 
ADVLNTLVYR PQCIETTALG AAYLAGLATG YFKDRNEIKD NWTLGGTFSP QMDEQKRKEL 

       490 
LKGWKRAVRC ALAWAEDIE 

« Hide

References

[1]"Complete genome sequence of Clostridium phytofermentans ISDg."
Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L., LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C. expand/collapse author list , Han C., Kuske C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.A., Richardson P.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700394 / DSM 18823 / ISDg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000885 Genomic DNA. Translation: ABX40431.1.
RefSeqYP_001557170.1. NC_010001.1.

3D structure databases

ProteinModelPortalA9KQC2.
SMRA9KQC2. Positions 2-496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING357809.Cphy_0041.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX40431; ABX40431; Cphy_0041.
GeneID5744961.
KEGGcpy:Cphy_0041.
PATRIC19499534. VBICloPhy16213_0040.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0554.
HOGENOMHOG000222134.
KOK00864.
OMAVYGTTRG.
OrthoDBEOG6RZB46.
ProtClustDBCLSK2796248.

Enzyme and pathway databases

BioCycCPHY357809:GHCL-52-MONOMER.
UniPathwayUPA00618; UER00672.

Family and domain databases

HAMAPMF_00186. Glycerol_kin.
InterProIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01311. glycerol_kin. 1 hit.
PROSITEPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPK_CLOPH
AccessionPrimary (citable) accession number: A9KQC2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: February 19, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways