A9KP85 (A9KP85_CLOPH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase RuleBase RU000515
EC=4.2.1.24 RuleBase RU000515

Gene names

Ordered Locus Names:

Cphy_1372

Organism

Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) [Complete proteome] [HAMAP]

Taxonomic identifier

357809 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O. RuleBase RU000515
Subunit structure	Homooctamer By similarity. PIRSR PIRSR001415-3 RuleBase RU000515
Sequence similarities	Belongs to the ALADH family. RuleBase RU004161

Ontologies

Keywords
Biological process	Porphyrin biosynthesis RuleBase RU000515
Ligand	Magnesium PIRSR PIRSR001415-5 Metal-binding PIRSR PIRSR001415-5 Zinc PIRSR PIRSR001415-3
Molecular function	Lyase RuleBase RU000515 EMBL ABX41747.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	198	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Active site	251	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Metal binding	123	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	125	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	133	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	236	1	Magnesium By similarity PIRSR PIRSR001415-5
	Binding site	208	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	220	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	277	1	Substrate 2 By similarity PIRSR PIRSR001415-2
	Binding site	316	1	Substrate 2 By similarity PIRSR PIRSR001415-2

Sequences

	Sequence		Length	Mass (Da)
	A9KP85 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 572C3D28F78AE1A5	FASTA	331	36,883
	10 20 30 40 50 60 MAVKMDMIQR PRRLRNNSIL RKMVRETRMD KSSLVYPVFI KGGVGIEEEI KSMEGQFRYS 70 80 90 100 110 120 IDRLPYKLEE LAKAGIDKVL LFGIPEEKDE CGSSAFDDNG IIQKALRSLK MQFPEMYCIT 130 140 150 160 170 180 DVCMCEYTSH GHCGILNGDK VDNDKTLEYL AKIALSHAEA GADMIAPSDM MDGRIGAIRR 190 200 210 220 230 240 MLDSNQLEHI PIMSYAVKYA SAFYGPFREA AGSAPAFGDR KTYQMDYHNK REAMKEALLD 250 260 270 280 290 300 IEEGADIIMV KPALSYHDVI NEVSNAVQVP VAAYSVSGEY AMVKAAAKAG FINEADVICE 310 320 330 MATGAYRAGA SIYITYFANE LARYMDEGRI G

References

[1]

"Complete genome sequence of Clostridium phytofermentans ISDg." Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L., LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C. , Han C., Kuske C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.A., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000885 Genomic DNA. Translation: ABX41747.1.
RefSeq	YP_001558486.1. NC_010001.1.
3D structure databases
ProteinModelPortal	A9KP85.
SMR	A9KP85. Positions 8-325.
ModBase	Search...
Protein-protein interaction databases
STRING	A9KP85.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5742322.
GenomeReviews	Gene locus Cphy_1372 in contig CP000885_GR.
KEGG	cpy:Cphy_1372.
PATRIC	19502366. VBICloPhy16213_1422.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285270.
OMA	ADLCFCE.
ProtClustDB	PRK09283.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01698.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A9KP85_CLOPH

Accession

Primary (citable) accession number: A9KP85

Entry history

Integrated into UniProtKB/TrEMBL:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)