Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A9KNJ5 (FTHS_CLOPH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Cphy_2752
OrganismClostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) [Complete proteome] [HAMAP]
Taxonomic identifier357809 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000333313

Regions

Nucleotide binding65 – 728ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9KNJ5 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 39973DD253DFBAE8

FASTA55660,275
        10         20         30         40         50         60 
MKTDIEIAQE AKMIHIREVA KSLGITEDDL DFYGKYKAKF TDELWDEIKD REDGKLILVT 

        70         80         90        100        110        120 
AINPTPAGEG KTTTTVGLGQ AFGKLEKNAV IALREPSLGP CFGIKGGAAG GGYAQVVPME 

       130        140        150        160        170        180 
DLNLHFTGDF HAITSANNLL AAMLDNHIQQ GNTLGIDTNQ IVWKRCVDMN DRVLRNIVVG 

       190        200        210        220        230        240 
LGRKADGVVR EDHFIITVAS EIMAILCLAS DMNDLKERLS RIIVAYSYEG KPITAKDLHA 

       250        260        270        280        290        300 
VGSMAALLKD AIRPNLIQTL ENTPAIIHGG PFANIAHGCN SVRATKTALK LADYVITEAG 

       310        320        330        340        350        360 
FGADLGAEKF LDIKCRIADL KPAAVVLVAT IRALKYNGGV AKTDLSSENL EALEKGIVNL 

       370        380        390        400        410        420 
EKHIENIQKF GVPVVVTLNK FSTDTERELS YVKQFCEQRG CEFSLSEVWE KGGEGGIDLA 

       430        440        450        460        470        480 
NKVIKTIETK ESNYHVLYPN EMSLKEKMGT IAKEIYGADG VTFDSGALKE VERLTELGFG 

       490        500        510        520        530        540 
NLPVCMAKNQ YSLSDDPSKL GRPTNFTVNI REVYVSAGAG FVVAITGTVM TMPGLPKVPA 

       550 
AEHIDVNEEG VITGLF 

« Hide

References

[1]"Complete genome sequence of Clostridium phytofermentans ISDg."
Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L., LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C. expand/collapse author list , Han C., Kuske C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.A., Richardson P.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700394 / DSM 18823 / ISDg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000885 Genomic DNA. Translation: ABX43112.1.
RefSeqYP_001559851.1. NC_010001.1.

3D structure databases

ProteinModelPortalA9KNJ5.
SMRA9KNJ5. Positions 4-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING357809.Cphy_2752.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX43112; ABX43112; Cphy_2752.
GeneID5742067.
KEGGcpy:Cphy_2752.
PATRIC19505260. VBICloPhy16213_2866.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMADPKKINA.
OrthoDBEOG6PCPSP.

Enzyme and pathway databases

BioCycCPHY357809:GHCL-2794-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_CLOPH
AccessionPrimary (citable) accession number: A9KNJ5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways