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Protein

16S rRNA (cytosine(967)-C(5))-methyltransferase

Gene

Cphy_2489

Organism
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.SAAS annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.SAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei292S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei319S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei337S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei390NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferasePROSITE-ProRule annotationSAAS annotation, RNA-bindingPROSITE-ProRule annotationSAAS annotation, Transferase
Biological processrRNA processingSAAS annotation
LigandS-adenosyl-L-methioninePROSITE-ProRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
16S rRNA (cytosine(967)-C(5))-methyltransferaseSAAS annotation (EC:2.1.1.176SAAS annotation)
Gene namesi
Ordered Locus Names:Cphy_2489Imported
OrganismiLachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)Imported
Taxonomic identifieri357809 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceae
Proteomesi
  • UP000000370 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm SAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi357809.Cphy_2489.

Structurei

3D structure databases

ProteinModelPortaliA9KM96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 453SAM_MT_RSMB_NOPInterPro annotationAdd BLAST282

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000037300.
KOiK03500.
OMAiLRVNRQH.
OrthoDBiPOG091H02E2.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
InterProiView protein in InterPro
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiView protein in PROSITE
PS51686. SAM_MT_RSMB_NOP. 1 hit.

Sequencei

Sequence statusi: Complete.

A9KM96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAREIVLTM CLKINEDKLP SHVVINETLK NYKDLSKQDR AFISRLCLGV
60 70 80 90 100
LEQKLRLDYD LERFSKIKVK KMKPVIRNVL RIAAYQIEFM EQIPDSAACD
110 120 130 140 150
EAVKLVKKRG LGGLSGFVNG ILRNLSREFQ KANGFDISKL NEIERLSYQY
160 170 180 190 200
SMPKELVEFW LKSFRREQVE EMLQAFLEPK DTSIRINESK ITIDELVDNL
210 220 230 240 250
VKDGVEVRDG AYLTNAKRIT SYNSLPVLDS FQKGYFQVQD ESSMMVGLIA
260 270 280 290 300
GIKAGDYIID VCAAPGGKTL HAADLLLVAG GEKNPGHVDA RDKTDRKVAL
310 320 330 340 350
IEQNLERIGY QNVSVKVFDA LVPDDSVKEK ADIILADLPC SGLGVIGKKP
360 370 380 390 400
DIKYNMTKEM MSELAKLQRN ILTVVVSYLK PGGVLIFSTC TINPEENEDN
410 420 430 440 450
IRFITETLKL QPESIDTYLP ENLQCETSKQ GYLQLLPNKS GVDGFFISRF

RKV
Length:453
Mass (Da):51,132
Last modified:February 5, 2008 - v1
Checksum:iD8D8D60050938619
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000885 Genomic DNA. Translation: ABX42850.1.
RefSeqiWP_012200503.1. NC_010001.1.

Genome annotation databases

EnsemblBacteriaiABX42850; ABX42850; Cphy_2489.
KEGGicpy:Cphy_2489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000885 Genomic DNA. Translation: ABX42850.1.
RefSeqiWP_012200503.1. NC_010001.1.

3D structure databases

ProteinModelPortaliA9KM96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi357809.Cphy_2489.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX42850; ABX42850; Cphy_2489.
KEGGicpy:Cphy_2489.

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000037300.
KOiK03500.
OMAiLRVNRQH.
OrthoDBiPOG091H02E2.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
InterProiView protein in InterPro
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiView protein in PROSITE
PS51686. SAM_MT_RSMB_NOP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA9KM96_LACP7
AccessioniPrimary (citable) accession number: A9KM96
Entry historyiIntegrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: June 7, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.