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A9KL30 (A9KL30_CLOPH) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS HAMAP MF_02003
Ordered Locus Names:Cphy_3832
OrganismClostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) [Complete proteome] [HAMAP]
Taxonomic identifier357809 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length1091 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
A9KL30 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: FAF16147BE8A42CC

FASTA1,091124,672
        10         20         30         40         50         60 
MYEKVSTNLN FVDREKEVLK FWKENEIFEK SIDSRKEGET FTFYDGPPTA NGKPHIGHVL 

        70         80         90        100        110        120 
TRVIKDMIPR YRTMKGCMVP RKAGWDTHGL PVELEVEKLL ELNGKEQIEQ YGLEPFITKC 

       130        140        150        160        170        180 
KESVWKYKGM WEDFSGTVGF WADMDDPYVT YHNDYIESVW WSLKQVWDKG LLYKGYKIVP 

       190        200        210        220        230        240 
YCPRCGTPLA SHEVAQGYKD VKEKSVIAKF RVMDSANEYF LAWTTTPWTL PSNVALCVNP 

       250        260        270        280        290        300 
KEDYVKVEVS VNAKGNVVKS PKGEEEVTEE HTAVGVEYYI LAEALCSVIE GDYRVVESYK 

       310        320        330        340        350        360 
GSDLEYMKYD PLFDYAYNNA DGTRNEKANR NNVNGKKAYY VTCADYVTLT DGTGIVHIAP 

       370        380        390        400        410        420 
AFGADDANVG NRYELPFVQL VDEKGQMKAE VTDFAGLFCK KSDEGILKKL GETKQLFKVL 

       430        440        450        460        470        480 
KFEHSYPFCW RCDTPLIYYA RESWFIKMTA VKDKMIANNN TINWIPETIG KGRFGDWLEN 

       490        500        510        520        530        540 
LQDWGISRNR YWGTPLNVWT CDCGKMHSIG SIEELKSMSK NCPEDIELHR PYIDNVTITC 

       550        560        570        580        590        600 
PDCGKEMHRV PEVIDCWYDS GAMPFAQHHY PFENKEKFEA QFPADFISEA VDQTRGWFYS 

       610        620        630        640        650        660 
LLAISTLIFD KAPYKNVIVL GHVQDENGQK MSKSKGNAVD PFDALEEYGA DAIRWYFYIN 

       670        680        690        700        710        720 
SAPWLPNRFH GKAVVEGQRK FMGTLWNTYA FFVLYANIDQ FDATKYSLDY DKLPVMDKWL 

       730        740        750        760        770        780 
LSKLNTVVKT VDENLAAYKI PETARALQEF VDELSNWYVR RGRERFWAKG MEQDKINAYM 

       790        800        810        820        830        840 
TLYTALVTIA KASAPMVPFM AEDIYRNLVC SIDKNAPLSV HLCDFPTWDA KLIDTKLEAD 

       850        860        870        880        890        900 
MEAVLEIVVL GRACRNTANI KQRQPISKMY VKAELTYTAN DGSVVEGMSD FYKEIIEDEL 

       910        920        930        940        950        960 
NVKNVVFTSD VRNFTSYTFK PQLKTLGRRF GSKLNALKEV LSSLNGNETM DELNEKGTLT 

       970        980        990       1000       1010       1020 
ITLDGTEEVL EKDDLLIDAA QMEGYVTDSD YGITVVLDTN LTEELIEEGF VREVISKVQT 

      1030       1040       1050       1060       1070       1080 
MRKDAGFEVM DHIVVSVKDN EKVEAIVKKN VAEISSIVLA DDVVYGEAQG FSKEWNLNGE 

      1090 
DVTLGVMKVN A

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References

[1]"Complete genome sequence of Clostridium phytofermentans ISDg."
Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L., LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C. expand/collapse author list , Han C., Kuske C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.A., Richardson P.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000885 Genomic DNA. Translation: ABX44179.1.
RefSeqYP_001560918.1. NC_010001.1.

3D structure databases

ProteinModelPortalA9KL30.
ModBaseSearch...

Protein-protein interaction databases

STRINGA9KL30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5744784.
GenomeReviewsGene locus Cphy_3832 in contig CP000885_GR.
KEGGcpy:Cphy_3832.
PATRIC19507575. VBICloPhy16213_3997.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG577712.
OMAQFGKWLE.
ProtClustDBPRK06039.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA9KL30_CLOPH
AccessionPrimary (citable) accession number: A9KL30
Entry history
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info