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A9KKH7 (A9KKH7_CLOPH) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tyrosine--tRNA ligase HAMAP-Rule MF_02007
EC=6.1.1.1 HAMAP-Rule MF_02007
Alternative name(s):
Tyrosyl-tRNA synthetase HAMAP-Rule MF_02007
Gene names
Name:tyrS HAMAP-Rule MF_02007
Ordered Locus Names:Cphy_0761
OrganismClostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) [Complete proteome] [HAMAP] EMBL ABX41148.1
Taxonomic identifier357809 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium ›

Protein attributes

Sequence length402 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP-Rule MF_02007 SAAS SAAS002307

Catalytic activity

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP-Rule MF_02007 SAAS SAAS002307

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_02007 SAAS SAAS002307

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02007.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. HAMAP-Rule MF_02007

Contains 1 S4 RNA-binding domain. HAMAP-Rule MF_02007 SAAS SAAS002307

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain342 – 40261S4 RNA-binding By similarity HAMAP-Rule MF_02007
Motif46 – 5510"HIGH" region By similarity HAMAP-Rule MF_02007
Motif230 – 2345"KMSKS" region By similarity HAMAP-Rule MF_02007

Sites

Binding site2331ATP By similarity HAMAP-Rule MF_02007

Sequences

Sequence LengthMass (Da)Tools
A9KKH7 [UniParc].

Last modified February 5, 2008. Version 1.
Checksum: 14734ECEE2968162

FASTA40245,838
        10         20         30         40         50         60 
MICVDEQIKI IKKGVLDMMR EEELREKLKV SVEQNNPLTI KLGLDPSAPD IHLGHTVVLR 

        70         80         90        100        110        120 
KIKQMQDLGH RAIIIIGDFT GRIGDPTGKS KTRKPLTDEQ VKENAATYME QIFKVLDREK 

       130        140        150        160        170        180 
TEVRFNSEWL SKLNFEQVIS LASSMTVARL LERDDFQNRY KNQEAIGLHE FFYPLMQGYD 

       190        200        210        220        230        240 
SVEIKADIEL GGTDQTFNIL MGRTLQKSVG MSQQIAMFMP ILEGLDGIEK MSKSLGNYIG 

       250        260        270        280        290        300 
VSESPQVMFK KVMEVPDQLI FRYFELVTDE HPDDIEKLRI EMEQGKNPRD IKLILARIIT 

       310        320        330        340        350        360 
NLYHGEEGAK AGEEYFEQVF KEGEIPEDMP VIKLEKEQDS LASVAGVLAS EGLVPSASEF 

       370        380        390        400 
RRLIKQGGVQ LNREKVLDID CVLTEQENVL KIGKKKFVRL VF

« Hide

References

[1]"Complete genome sequence of Clostridium phytofermentans ISDg."
Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L., LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C. expand/collapse author list , Han C., Kuske C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.A., Richardson P.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700394 / DSM 18823 / ISDg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000885 Genomic DNA. Translation: ABX41148.1.
RefSeqYP_001557887.1. NC_010001.1.

3D structure databases

ProteinModelPortalA9KKH7.
ModBaseSearch...

Protein-protein interaction databases

STRING357809.Cphy_0761.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX41148; ABX41148; Cphy_0761.
GeneID5743877.
KEGGcpy:Cphy_0761.
PATRIC19501092. VBICloPhy16213_0788.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0162.
HOGENOMHOG000242791.
KOK01866.
OMANIAIMEP.
ProtClustDBPRK13354.

Enzyme and pathway databases

BioCycCPHY357809:GHCL-849-MONOMER.

Family and domain databases

Gene3D3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_02007. Tyr_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024108. Tyr-tRNA-ligase_bac_2.
[Graphical view]
PANTHERPTHR11766. PTHR11766. 1 hit.
PfamPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01040. TRNASYNTHTYR.
SMARTSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00234. tyrS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA9KKH7_CLOPH
AccessionPrimary (citable) accession number: A9KKH7
Entry history
Integrated into UniProtKB/TrEMBL: February 5, 2008
Last sequence update: February 5, 2008
Last modified: May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info