A9KHV5 (A9KHV5_CLOPH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase HAMAP MF_00555
EC=6.3.1.1 HAMAP MF_00555

Alternative name(s):
Asparagine synthetase A HAMAP MF_00555

Gene names

Name:	asnA HAMAP MF_00555
Ordered Locus Names:	Cphy_3449

Organism

Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) [Complete proteome] [HAMAP]

Taxonomic identifier

357809 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555 SAAS SAAS004618
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555 SAAS SAAS004618
Subcellular location	Cytoplasm By similarity HAMAP MF_00555 SAAS SAAS004618.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily. HAMAP MF_00555

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis HAMAP MF_00555 SAAS SAAS004618
Cellular component	Cytoplasm HAMAP MF_00555 SAAS SAAS004618
Ligand	ATP-binding HAMAP MF_00555 SAAS SAAS004618 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00555 SAAS SAAS004618 EMBL ABX43802.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A9KHV5 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: FAAEAF9265FE9B1D	FASTA	337	38,406
	10 20 30 40 50 60 MLELLILPKD YHSALDIKDT QGAIKHVKDH FERQLAKQLN LTRVSAPLFV RPETGLNDNL 70 80 90 100 110 120 SGVERPVSFG VKEQDDAEVE IVHSLAKWKR MALKKYGFAY GEGLYTDMNA IRRDEDTDNI 130 140 150 160 170 180 HSIFVDQWDW EKILLHDERN VETLKSVVRS VYQALKDTEH YISDKYDYIG KILPKEIAFV 190 200 210 220 230 240 TTQELENMYP NDTPKDREYK IAKLKGAVFI MQIGGALASG EKHDGRAPDY DDWSLNGDII 250 260 270 280 290 300 VYYPVLDIAL ELSSMGIRVD ETSLANQLEI ADCKDRAKLP FQKAVLNREL PYTVGGGIGQ 310 320 330 SRICMFFLRK AHIGEVQSSI WKEEDIEKLG NNGITLL

References

[1]

"Complete genome sequence of Clostridium phytofermentans ISDg." Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L., LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C. , Han C., Kuske C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.A., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000885 Genomic DNA. Translation: ABX43802.1.
RefSeq	YP_001560541.1. NC_010001.1.
3D structure databases
ProteinModelPortal	A9KHV5.
ModBase	Search...
Protein-protein interaction databases
STRING	A9KHV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5743561.
GenomeReviews	Gene locus Cphy_3449 in contig CP000885_GR.
KEGG	cpy:Cphy_3449.
PATRIC	19506778. VBICloPhy16213_3603.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A9KHV5_CLOPH

Accession

Primary (citable) accession number: A9KHV5

Entry history

Integrated into UniProtKB/TrEMBL:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)