A9KHU4 (TGT_CLOPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29

Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase

Gene names

Name:	tgt
Ordered Locus Names:	Cphy_3438

Organism

Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) [Complete proteome] [HAMAP]

Taxonomic identifier

357809 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP MF_00168
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00168
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_1000077002
Sites
	Active site	89	1	Nucleophile By similarity
	Metal binding	309	1	Zinc By similarity
	Metal binding	311	1	Zinc By similarity
	Metal binding	314	1	Zinc By similarity
	Metal binding	340	1	Zinc By similarity
	Binding site	90	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9KHU4 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 6A74358309962209	FASTA	378	42,718
	10 20 30 40 50 60 MYKLICKDGN AKRGEFTTVH GKIQTPVFMN VGTAAAIKGA VSTMDLQEIG TQVELSNTYH 70 80 90 100 110 120 LHVRPGDEVV KKLGGLHKFM VWDKPILTDS GGFQVFSLAG LRKIKEEGVY FNSHIDGKKI 130 140 150 160 170 180 FMGPEESMRI QSNLASTIAM AFDECPPHPA TREYMEDSVA RTTRWLLRCK NEMNRLNTLE 190 200 210 220 230 240 DTINKHQMLF GINQGGTYTD IRVEHAKRIS ELDLDGYALG GLAVGESHSE MYRIIEETVP 250 260 270 280 290 300 YLPEAKPTYL MGVGTPANIL EAVERGVDFF DCVYPARNGR HGHAYTNHGK MNLLNAKYEL 310 320 330 340 350 360 DDRPIEEGCG CPVCKNYSRG YIRHLLKAKE MLGLRFLVTH NLYFYNKMME EIREAIENQN 370 FASYKKKKLE GFAAEQGN

References

[1]

"Complete genome sequence of Clostridium phytofermentans ISDg." Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L., LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C. , Han C., Kuske C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.A., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700394 / DSM 18823 / ISDg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000885 Genomic DNA. Translation: ABX43791.1.
RefSeq	YP_001560530.1. NC_010001.1.
3D structure databases
ProteinModelPortal	A9KHU4.
ModBase	Search...
Protein-protein interaction databases
STRING	A9KHU4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5743550.
GenomeReviews	Gene locus Cphy_3438 in contig CP000885_GR.
KEGG	cpy:Cphy_3438.
PATRIC	19506752. VBICloPhy16213_3590.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG629929.
OMA	FMPVGTV.
ProtClustDB	PRK00112.
Enzyme and pathway databases
BioCyc	CPHY357809:CPHY_3438-MONOMER.
Family and domain databases
HAMAP	MF_00168. Q_tRNA_Tgt. [Tree]
InterPro	IPR004803. Queuine_tRNA-ribosylTrfase. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
KO	K00773.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
SUPFAM	SSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. Tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_CLOPH

Accession

Primary (citable) accession number: A9KHU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program