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Protein

Argininosuccinate synthase

Gene

argG

Organism
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86CitrullineUniRule annotation1
Binding sitei91CitrullineUniRule annotation1
Binding sitei116ATP; via amide nitrogenUniRule annotation1
Binding sitei118AspartateUniRule annotation1
Binding sitei122AspartateUniRule annotation1
Binding sitei122CitrullineUniRule annotation1
Binding sitei123AspartateUniRule annotation1
Binding sitei126CitrullineUniRule annotation1
Binding sitei178CitrullineUniRule annotation1
Binding sitei187CitrullineUniRule annotation1
Binding sitei264CitrullineUniRule annotation1
Binding sitei276CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 16ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Cphy_1931
OrganismiLachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)
Taxonomic identifieri357809 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceae
Proteomesi
  • UP000000370 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003294681 – 407Argininosuccinate synthaseAdd BLAST407

Proteomic databases

PRIDEiA9KHL4.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi357809.Cphy_1931.

Structurei

3D structure databases

ProteinModelPortaliA9KHL4.
SMRiA9KHL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiGVGRIDM.
OrthoDBiPOG091H01U0.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR11587. PTHR11587. 1 hit.
PfamiView protein in Pfam
PF00764. Arginosuc_synth. 1 hit.
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiView protein in PROSITE
PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A9KHL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEKVILAYS GGLDTTAIIP WLKENYDYDV VCCCIDVGQG NELDGLEERA
60 70 80 90 100
KLSGATKLYI EHVTDEFVDE YVMPCVKAGA VYENKYLLGT SMARPVIAKK
110 120 130 140 150
LVEVAIKENA VAICHGATGK GNDQVRFELG IKALAPDLKI IAPWRCNNTW
160 170 180 190 200
KMQSREDEIE YCKAHGIDLP FDASHSYSRD RNLWHISHEG LELEDPANAP
210 220 230 240 250
NYDHLLVLGV SPEKAPNEAE SLSLTFEKGI PVALNGKAMK ASEIIEELNV
260 270 280 290 300
LGGKYGIGII DIVENRVVGM KSRGVYETPG GTILMEAHTQ LEELILDRAT
310 320 330 340 350
LSCKKEIGNK FADVVYEGKW FTPLREALQA FVEVTQEYVT GEVKLKLYKG
360 370 380 390 400
NIIKQGTTSP YSLYNESIAS FTTGELYNHH DAEGFINLFG LSLKVRAMKM

AEVEKNK
Length:407
Mass (Da):45,218
Last modified:February 5, 2008 - v1
Checksum:i5DD540F4E211BAE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000885 Genomic DNA. Translation: ABX42299.1.

Genome annotation databases

EnsemblBacteriaiABX42299; ABX42299; Cphy_1931.
KEGGicpy:Cphy_1931.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiASSY_LACP7
AccessioniPrimary (citable) accession number: A9KHL4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 5, 2008
Last modified: June 7, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families