A9KFT9 (MDH_COXBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	CBUD_1325

Organism

Coxiella burnetii (strain Dugway 5J108-111) [Complete proteome] [HAMAP]

Taxonomic identifier

434922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517
Subunit structure	Homodimer By similarity. HAMAP MF_01517
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Malate dehydrogenase HAMAP MF_01517		PRO_1000087542
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
	Nucleotide binding	129 – 131	3	NAD By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	105	1	NAD By similarity
	Binding site	112	1	NAD By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9KFT9 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 0B74C2B5DE757FD8	FASTA	328	35,396
	10 20 30 40 50 60 MAKHVKVAVT GAAGQIGYAL LFRLASGQAF GLDTTVDLHL LEIEPALPAL KGVVTELEDC 70 80 90 100 110 120 AFPLLCNMVV TSDPRVAFND VNWALLVGAA PRKAGMERKD LLEKNGSIFA GQGKAINENA 130 140 150 160 170 180 ASDVRIFVVG NPCNTNCLIA MNNAPDIPKD RFYAMTRLDQ NRAIGQLALK AGVDVPSVKN 190 200 210 220 230 240 MIIWGNHSST QYPDFYHATI DGKPATEVIR DKNWLLNDFI PVIQQRGAAV IKARGASSAA 250 260 270 280 290 300 SAANAALDSV WSLINTTPAD DNYSVALCAQ GQYGVDEGLI FSFPCRTENG VVSVIEEIEH 310 320 NEFGQQKLKE TLDELREERD AVEALGLI

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed: 19047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Dugway 5J108-111.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000733 Genomic DNA. Translation: ABS77292.1.
RefSeq	YP_001424678.1. NC_009727.1.
3D structure databases
ProteinModelPortal	A9KFT9.
SMR	A9KFT9. Positions 1-328.
ModBase	Search...
Protein-protein interaction databases
STRING	A9KFT9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5459058.
GenomeReviews	Gene locus CBUD_1325 in contig CP000733_GR.
KEGG	cbd:CBUD_1325.
PATRIC	17922479. VBICoxBur32972_1332.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289884.
OMA	NKIQISL.
ProtClustDB	PRK05442.
Enzyme and pathway databases
BioCyc	CBUR434922:COXBU7E912_1325-MONOMER.
Family and domain databases
HAMAP	MF_01517. Malate_dehydrog_2. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00024.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_COXBN

Accession

Primary (citable) accession number: A9KFT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program