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A9KFK6

- PNP_COXBN

UniProt

A9KFK6 - PNP_COXBN

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, CBUD_0917
Organism
Coxiella burnetii (strain Dugway 5J108-111)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi489 – 4891Magnesium By similarity
Metal bindingi495 – 4951Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCBUR434922:GJTP-976-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:CBUD_0917
OrganismiCoxiella burnetii (strain Dugway 5J108-111)
Taxonomic identifieri434922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000008555: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 696696Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329613Add
BLAST

Proteomic databases

PRIDEiA9KFK6.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

STRINGi434922.CBUD_0917.

Structurei

3D structure databases

ProteinModelPortaliA9KFK6.
SMRiA9KFK6. Positions 620-694.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini556 – 61560KH
Add
BLAST
Domaini625 – 69369S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9KFK6-1 [UniParc]FASTAAdd to Basket

« Hide

MNKIRKTFQY GKHEVTFETG EMARQATGAV VVRMGDTVLL VSVVAKKEAE    50
EGRDFFPLTV NYQEKTYAAG KIPGGYFKRE GRPTEKETLT SRLIDRPLRP 100
LFPKGFTNEV QVIATVLSVD SKVPTDIPAI LGASAAIGLS GIPFNGSLGA 150
ARVGYRGGEY LLNPSLDELK DSALDLVVAG TRDAVLMVES EAQELPESVM 200
LGAVLHGHQA MQVAIQAIAE FIQEAGGAKW EWEPPTVNTA LEKWVVEKSE 250
APLKKAYQIQ EKTARQAQIQ AIRDQLLADR AAEREGEENA VNEHELAVIF 300
HELERRIVRE QILTGQPRID GRDTKTVRPI TVKVGVLPRS HGSALFTRGE 350
TQALVVTTLG TERDAQSIDD LDGDRQEEFI FHYNFPPFCV GEVGFMSGPK 400
RREIGHGRLA KRAVVPVVPT LDKFPYVIRV VSEILESNGS SSMASVCGSS 450
LALMDAGVPT KAPVAGIAMG LIKENDKYAV LSDILGDEDH LGDMDFKVAG 500
TSNGVTALQM DIKIEGITKE IMEQALDQAK EGRLHILSIM NKVLDKPRSQ 550
VSDLAPQYVT MKINPEKIRD VIGKGGVVIR EITEATNCAI DISDDGTIKI 600
AAHTTEEGEA AKRRIEELTA EVELGKVYEG TVVKITDFGA FVQILPNTQG 650
LVHISQIAQE RVENVRDYLE EGQVIRVKVI EIDRQGRVRL SMKQID 696
Length:696
Mass (Da):76,331
Last modified:February 5, 2008 - v1
Checksum:i9E55E7C7CD00E52B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000733 Genomic DNA. Translation: ABS76705.1.
RefSeqiWP_010957848.1. NC_009727.1.
YP_001424300.1. NC_009727.1.

Genome annotation databases

EnsemblBacteriaiABS76705; ABS76705; CBUD_0917.
GeneIDi5457970.
KEGGicbd:CBUD_0917.
PATRICi17921676. VBICoxBur32972_0944.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000733 Genomic DNA. Translation: ABS76705.1 .
RefSeqi WP_010957848.1. NC_009727.1.
YP_001424300.1. NC_009727.1.

3D structure databases

ProteinModelPortali A9KFK6.
SMRi A9KFK6. Positions 620-694.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 434922.CBUD_0917.

Proteomic databases

PRIDEi A9KFK6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS76705 ; ABS76705 ; CBUD_0917 .
GeneIDi 5457970.
KEGGi cbd:CBUD_0917.
PATRICi 17921676. VBICoxBur32972_0944.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CBUR434922:GJTP-976-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella."
    Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A.
    Infect. Immun. 77:642-656(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Dugway 5J108-111.

Entry informationi

Entry nameiPNP_COXBN
AccessioniPrimary (citable) accession number: A9KFK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 5, 2008
Last modified: September 3, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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