Reviewed, UniProtKB/Swiss-Prot A9KEP6 (QUEF_COXBN)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	CBUD_1955

Organism

Coxiella burnetii (strain Dugway 5J108-111) [Complete proteome] [HAMAP]

Taxonomic identifier

434922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP cyclohydrolase I activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000083824

Sequences

	Sequence		Length	Mass (Da)
	A9KEP6-1 [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 0B41943AA19EEC99	FASTA	278	31,990
	10 20 30 40 50 60 MSTLRVLHEK SELGKTTVYP KEYAPHLLLP IPRDLNRKTL NVNVSEPPPF YGYDLWNAYE 70 80 90 100 110 120 LSWLNEKGKP FAARGEFIIP ATSSHLIESK SFKLYLNSFN NERFADAAAV SQTMKRDLSK 130 140 150 160 170 180 RVNESVTVNF ILHETEIPVA YSPKGSLLDV LDIAIDTYSP DPNLLSTSQE TVTETLYSHL 190 200 210 220 230 240 LKSNCPVTGQ PDWGSIEIHY TGPKIDHAQL LKYIISYRNH EEFHEACVER FFMDILRHCR 250 260 270 PQELTVQARY TRRGGLDINP YRSTNPTFSV QNHRSFRQ

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed: 19047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000733 Genomic DNA. Translation: ABS78127.1.
RefSeq	YP_001425265.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5457654.
GenomeReviews	Gene locus CBUD_1955 in contig CP000733_GR.
KEGG	cbd:CBUD_1955.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A9KEP6. EHNEFHE.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR001474. GTP_CycHdrlase_I. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_COXBN

Accession

Primary (citable) accession number: A9KEP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	June 16, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)