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A9KEI8

- SYI_COXBN

UniProt

A9KEI8 - SYI_COXBN

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Coxiella burnetii (strain Dugway 5J108-111)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
Binding sitei605 – 6051ATPUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi919 – 9191ZincUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCBUR434922:GJTP-1769-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CBUD_1672
OrganismiCoxiella burnetii (strain Dugway 5J108-111)
Taxonomic identifieri434922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000008555: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 936936Isoleucine--tRNA ligasePRO_1000088543Add
BLAST

Proteomic databases

PRIDEiA9KEI8.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi434922.CBUD_1672.

Structurei

3D structure databases

ProteinModelPortaliA9KEI8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

A9KEI8-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MTDYKDTLNL PQTDFPMRAN LPEREPQTLA RWQTLDLYRK IRKDREGQPK
60 70 80 90 100
FILHDGPPYA NGRAHLGTAF NKTLKDIVVK SKTLSGFDAP FVPGWDCHGL
110 120 130 140 150
PIELNVEKKL GKDKLSANAF RQACRDYAFS QIELQRDDFQ RLGVLGDWQH
160 170 180 190 200
PYLTMDFGYE ADTVRALAKI VANGHLLRGQ KPVHWCAACG SALAEAEVEY
210 220 230 240 250
RDKASPAVDV GFEAVDAEAV RQRFGVKNAT TRVLVPIWTT TPWTLPANEA
260 270 280 290 300
VSVHPELHYA LVKSELQNQP VYLILAKDLV DSAMQRYGVD DYEVHGNLKG
310 320 330 340 350
DALEGMQLQH PFLDRIVPII LGEHVTTEAG TGNVHTAPAH GLEDYFVAEK
360 370 380 390 400
YNLPINNPVD ARGRFIPDTF LVGGQPVFKA NEPIIVLLAD SGHLLHSETI
410 420 430 440 450
QHSYPHCWRH KTPLIFRTTP QWFIGMNKNG LRERALAEIE KVTWLPAWGE
460 470 480 490 500
ARIGKMVADR PDWCISRQRL WGIPIPLFIH KKSGELHPKS PALMEKVAQL
510 520 530 540 550
IEKESVDAWF DLDPKVLLGD DADHYEKVTD VLDVWFDSGV THFCVLEKRR
560 570 580 590 600
ELKVPADIYL EGSDQHRGWF QSSLLTSLAI RDKAPYKSVL TYGFVVDSQG
610 620 630 640 650
RKMSKSLGNV ILPADVVKNL GADVLRLWAA SMDYTVEVNV SDEILKRASD
660 670 680 690 700
AYRRIRNTAR FLLSNLYDFD PKKDKVAVDQ LVALDRWAIF TTQKLQEKII
710 720 730 740 750
TAYDRYRFPA IYQAIHNFCT VEMGSFYLDI IKDRLYTSKE SGLPRRSAQT
760 770 780 790 800
ALYYIAEAFV RWIAPIISFT ADEIWQFMPG DREPSVFLTQ WFSDFPNAAL
810 820 830 840 850
SGEEEQRWQL LLQIRDEVNK ALETYRNEGK IGSALAAEVV LYADERLNAA
860 870 880 890 900
IATLGEELRF VLITSEASVL PFNEKSKAAF DTALPGLALE INVSEFEKCA
910 920 930
RCWQRRSSVG QIKEHADLCD RCVSNAFEDG EMRQFA
Length:936
Mass (Da):106,140
Last modified:February 5, 2008 - v1
Checksum:i7AD669E4EA531C70
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000733 Genomic DNA. Translation: ABS78310.1.
RefSeqiWP_011997211.1. NC_009727.1.
YP_001425008.1. NC_009727.1.

Genome annotation databases

EnsemblBacteriaiABS78310; ABS78310; CBUD_1672.
GeneIDi5457682.
KEGGicbd:CBUD_1672.
PATRICi17923154. VBICoxBur32972_1666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000733 Genomic DNA. Translation: ABS78310.1 .
RefSeqi WP_011997211.1. NC_009727.1.
YP_001425008.1. NC_009727.1.

3D structure databases

ProteinModelPortali A9KEI8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 434922.CBUD_1672.

Proteomic databases

PRIDEi A9KEI8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS78310 ; ABS78310 ; CBUD_1672 .
GeneIDi 5457682.
KEGGi cbd:CBUD_1672.
PATRICi 17923154. VBICoxBur32972_1666.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CBUR434922:GJTP-1769-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella."
    Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A.
    Infect. Immun. 77:642-656(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Dugway 5J108-111.

Entry informationi

Entry nameiSYI_COXBN
AccessioniPrimary (citable) accession number: A9KEI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 5, 2008
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3