Reviewed, UniProtKB/Swiss-Prot A9KD88 (SAHH_COXBN)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	CBUD_2132

Organism

Coxiella burnetii (strain Dugway 5J108-111) [Complete proteome] [HAMAP]

Taxonomic identifier

434922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000129279
Regions
	Region	191 – 356	166	NAD binding By similarity
Sites
	Binding site	64	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	198	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9KD88-1 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: EF9BC88F64BA5F0F	FASTA	438	48,882
	10 20 30 40 50 60 MENDMETATM TQDYHIANIN LADWGRKEIE IAETEMPGLM ALRKKYKNAK PLKGARIAGC 70 80 90 100 110 120 IHMTIQTAVL IETLMLLGAE VRWSSCNIFS TQDHAAAALA QKGIPIFAWK GETEEEYWRC 130 140 150 160 170 180 IASTLEGPKG WTPNLLLDDG GDLTAHTLQK HPELCQNIRG VSEETTTGVH RLYRMLKEGS 190 200 210 220 230 240 LKFPAINVND SVTKSKFDNL YGCRESLIDS IKRATDVMIA GKRVVVCGYG DVGKGCAQSL 250 260 270 280 290 300 RAYGATVYIT EIDPICALQA AMEGYRVVTM DEMADSADIF VTATGNTDII THEHMLKMKD 310 320 330 340 350 360 QAIVCNIGHF DNEIDIASLQ DYQWMNIKPQ VDQVIFPDGK RLTVLAQGRL VNLGCATGHP 370 380 390 400 410 420 SFVMSNSFTN QVLAQIELWQ YPEKYPIGVY VLPKHLDEEV ARLHLERVGA KLTTLTEKQA 430 DYIGVDPEGP FKSEHYRY

References

[1]

"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella." Beare P.A., Unsworth N., Andoh M., Voth D.E., Omsland A., Gilk S.D., Williams K.P., Sobral B.W., Kupko J.J. III, Porcella S.F., Samuel J.E., Heinzen R.A. Infect. Immun. 77:642-656(2009) [PubMed: 19047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000733 Genomic DNA. Translation: ABS76996.2.
RefSeq	YP_001425436.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5458160.
GenomeReviews	Gene locus CBUD_2132 in contig CP000733_GR.
KEGG	cbd:CBUD_2132.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_COXBN

Accession

Primary (citable) accession number: A9KD88

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	September 22, 2009
This is version 19 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)